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  • C0979 - Anti-Catalase antibody ,Mouse monoclonal

C0979 Sigma-Aldrich

Anti-Catalase antibody ,Mouse monoclonal

clone CAT-505, purified from hybridoma cell culture



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies to Cell and Organelle Proteins,
conjugate   unconjugated
clone   CAT-505, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 0.5-1 μg/mL using a whole extract of HepG2 cells
species reactivity   bovine, human, mouse, rat
mol wt   antigen 60 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified from hybridoma cell culture
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
concentration   ~2 mg/mL
UniProt accession no.   P04040
Gene Information   human ... CAT(847)
mouse ... Cat(12359)
rat ... Cat(24248)


General description

Catalase is one of the most efficient and common enzyme found in nearly all living organisms exposed to oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). In most mammalian cell types catalase is localized in peroxisomes which is a tertrameric haemin-enzyme consisting of 4 identical tetrahedrally arranged subunits of 60kDa. It contains four ferriprotoporphyrin groups per molecule having a molecular mass of 240kDa.


human erythrocyte catalase


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (5 papers)

Monoclonal Anti-Catalase antibody produced in mouse is suitable for the following:
• Immunocytochemistry
• Immunohistochemistry
• Indirect ELISA
• Microarray
• Western blotting at a concentration of 0.5-1μg/mL using whole extract of HepG2 cells
• Immunofluorescence

Physical form

Solution in 0.01 M phosphate buffered saline, pH7.4, containing 15 mM sodium azide


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Catalase reacts with hydrogen peroxide, a reactive oxygen species, activating its decomposition into water and molecular oxygen and it reacts with hydrogen donors. It functions as a natural anti-oxidant, protecting cells against oxidative damage of proteins, lipids and nucleic acids. Catalase also plays a role in gene expression and apoptosis. Antioxidants have been identified in association with a variety of diverse cellular functions including growth control, proliferation, differentiation, immune response, tumor promotion, apoptosis and activation of viruses.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

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Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers


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