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  • C1041 - Monoclonal Anti-Desmosomal Cytokeratin antibody produced in mouse

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C1041 Sigma-Aldrich

Monoclonal Anti-Desmosomal Cytokeratin antibody produced in mouse

clone DK80.20, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytokeratins,
conjugate   unconjugated
clone   DK80.20, monoclonal
biological source   mouse
application(s)   electron microscopy: suitable
  immunohistochemistry (frozen sections): suitable
  indirect immunofluorescence: 1:100 using frozen bovine tissue sections
  western blot: suitable
species reactivity   bovine, human
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgM
antibody product type   primary antibodies
contains   15 mM sodium azide
UniProt accession no.   P05787
Gene Information   human ... KRT8(3856)

Description

Specificity

Reacts with cytokeratin peptide 8. The antibody detects desmosomes in frozen sections of bovine and human, non-stratified epithelium.

Immunogen

cytoskeletal proteins from the bovine mammary gland epithelial cell line BMGE

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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