C1129 Sigma-Aldrich

Cholic acid

from bovine and/or ovine, ≥98%

Synonym: 3α,7α,12α-Trihydroxy-5β-cholanic acid, Cholanic acid

  • CAS Number 81-25-4

  • Empirical Formula (Hill Notation) C24H40O5

  • Molecular Weight 408.57

  •  Beilstein/REAXYS Number 2822009

  •  EC Number 201-337-8

  •  MDL number MFCD00003672

  •  PubChem Substance ID 24892356



General description

Cholic acid is a major primary bile acid produced in the liver which facilitates fat absorption and cholesterol excretion. It is a non-denaturing ionic detergent.


Dietary cholic acid has been used in a study to assess its effect on colonic epithelial cell proliferation. It has also been used in a study to investigate extractions and comparisons of human erythrocyte ghosts.

Non-denaturing ionic detergent used for extraction of membrane proteins.


1 kg in poly bottle

25, 100, 500 g in poly bottle

Biochem/physiol Actions

Bile Acid

Other Notes

Tandem Mass Spectrometry data independently generated by Scripps Center for Metabolomics is available to view or download in PDF. C1129.pdf Tested metabolites are featured on Scripps Center for Metabolomics METLIN Metabolite Database. To learn more, visit

Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis

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Certificate of Origin

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Single- & multi-channel pipettes from BRAND
Protocols & Articles


Bile Acids

The liver excretes excess cholesterol in the form of bile acids. Bile acids serve two purposes: to remove unwanted cholesterol from the body and to aid in lipid digestion in the intestine. Bile acids...
BioFiles 2007, 2.7, 17.
Keywords: Absorption, Digestions, Transcription

Fast and Robust HPLC Separation of Bile Acids and Conjugates with Ascentis Express C18

Bile acids are increasingly recognized as playing an important signaling role in the control of immune response and energy metabolism. Recently, there has been interest in measuring bile acid concent...
Clare Glicksman, Michael Wright, Dave Bell, and Anders Fridström
Reporter US, Volume 33.1
Keywords: Bacterial conjugations, Clinical, High performance liquid chromatography, Hormones, Mass spectrometry, Metabolism, Separation, Thin layer chromatography

LC/MS Analysis of Bile Acids and Their Conjugates on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Bile Acids and Their Conjugates on Ascentis® Express C18
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry

MSMLS Online Plate Map

Metabolite descriptors included with the software download upon purchase of the product include: Name, Parent CID, molecular formula, molecular weight, CAS, ChEBI, HMDB ID, PubChem Compound and Subst...
Keywords: Mass spectrometry, Metabolites

Peer-Reviewed Papers


Related Products

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Product #


Add to Cart

614149 Cholic acid-2,2,4,4-d4, 98 atom % D, 98% (CP)
614106 Cholic acid-2,2,3,4,4-d5, 98 atom % D, 98% (CP)

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