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C-12710 Sigma-Aldrich

Human Chondrocytes (HCH)

500,000 cryopreserved cells

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Properties

Related Categories Cell Culture, Cells, Chondrocyte Cell Culture, Human Primary Cell Culture, Primary Cell Culture,
biological source   human articular cartilage (knee and hip joints)
packaging   pkg of 500,000 cells
morphology   (chondrocytes)
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Cartilage

Application

Primary Human Chondrocytes (HCH) are isolated from normal human articular cartilage from the knee and hip joints (lot specific source information is available on request) and are provided in a cryopreserved format. HCH are routinely tested for their capacity to differentiate in 3D spheroids.The articular cartilage covers the joints between bones and contains no blood vessels, lymphatic vessels, or nerve fibers. It is composed of only one specialized cell type, the chondrocytes, which produce and maintain the extracellular matrix of cartilage, i.e. collagen (mostly type II) and proteoglycans (primarily aggrecan).

Quality

Rigid quality control tests are performed for each lot of Human Chondrocytes. They are tested for cell morphology, adherence rate, and cell viability. Furthermore, each lot is routinely tested for the capacity to differentiate in 3D spheroids. Growth performance is tested through multiple passages up to 10 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV and HCV, and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

Click here for more information.

Other Notes

Recommended Plating Density: 10000 - 20000 cells per cm2Passage After Thawing: P2Tested Markers: Differentiation testedGuaranteed population doublings: > 10

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

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Protocols & Articles

Protocols

Primary Human Chondrocytes and Chondrocyte Growth Media

In the human body, articular cartilage covers the bones in the major joints, such as knee and hip joints. It is composed solely of chondrocytes, which are specialized cells that produce and maintain ...
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