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C-12750 Sigma-Aldrich

Human Chondrocytes (HCH)

500,000 proliferating cells



Related Categories Cell Culture, Cells, Chondrocyte Cell Culture, Human Primary Cell Culture, Primary Cell Culture,
biological source   human articular cartilage (knee and hip joints)
packaging   pkg of 500,000 cells
morphology   (chondrocytes)
application(s)   cell culture | mammalian: suitable
shipped in   ambient
storage temp.   room temp


Cell Line Origin



Primary Human Chondrocytes (HCH) are isolated from normal human articular cartilage from the knee and hip joints (lot specific source information is available on request) and are provided in a proliferating format. PromoCell HCH are routinely tested for their capacity to differentiate in 3D spheroids.The articular cartilage covers the joints between bones and contains no blood vessels, lymphatic vessels, or nerve fibers. It is composed of only one specialized cell type, the chondrocytes, which produce and maintain the extracellular matrix of cartilage, i.e. collagen (mostly type II) and proteoglycans (primarily aggrecan).


Rigid quality control tests are performed for each lot of Human Chondrocytes. They are tested for cell morphology, adherence rate, and cell viability. Furthermore, each lot is routinely tested for the capacity to differentiate in 3D spheroids. Growth performance is tested through multiple passages up to 10 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV and HCV, and microbial contaminants (fungi, bacteria, and mycoplasma).


Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

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Other Notes

Recommended Plating Density: 10000 - 20000 cells per cm2Passage After Thawing: P2Tested Markers: Differentiation testedGuaranteed population doublings: > 10

Safety & Documentation

Safety Information

WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Pipette Controller from Brand
Protocols & Articles


Primary Human Chondrocytes and Chondrocyte Growth Media

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