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C-14070 Sigma-Aldrich

Human Chondrocytes (HCH)

1 million cells per pellet

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Properties

Related Categories Cell Culture, Cells, Chondrocyte Cell Culture, Human Primary Cell Culture, Primary Cell Culture,
biological source   human articular cartilage (knee and hip joints)
packaging   pkg of 1,000,000 cells
morphology   (chondrocytes)
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −20°C

Description

Cell Line Origin

Cartilage

Application

Primary Human Chondrocytes (HCH) are isolated from normal human articular cartilage from the knee and hip joints (lot specific source information is available on request). PromoCell HCH are routinely tested for their capacity to differentiate in 3D spheroids.The articular cartilage covers the joints between bones and contains no blood vessels, lymphatic vessels, or nerve fibers. It is composed of only one specialized cell type, the chondrocytes, which produce and maintain the extracellular matrix of cartilage, i.e. collagen (mostly type II) and proteoglycans (primarily aggrecan).

Quality

Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

Click here for more information.

Other Notes

The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Pipette Controller from Brand
Protocols & Articles

Protocols

Primary Human Chondrocytes and Chondrocyte Growth Media

In the human body, articular cartilage covers the bones in the major joints, such as knee and hip joints. It is composed solely of chondrocytes, which are specialized cells that produce and maintain ...
Keywords: Antibiotics, Diagnostic, Gene expression, Phase transitions

PromoCell Primary Cell Pellets

PromoCell Cell Pellets are stored in RNAlater® reagent, an aqueous protection solution that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins and protects them from degradati...
Keywords: Degradations, Diagnostic, Electrophoresis, Gel electrophoresis, Gene expression, Western blot

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