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C1603 Sigma-Aldrich

Anti-Calcium Channel (α1C Subunit) (L-type of Voltage-gated Ca2+ Channel) antibody produced in rabbit

affinity isolated antibody

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Calcium Channels, Antibodies to Ion Channels,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: suitable using Rat brain sections
  western blot: 1:200 using Rat brain and rat heart membranes
species reactivity   rabbit, rat, mouse, human
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   100
antibody product type   primary antibodies
UniProt accession no.   Q13936
Gene Information   mouse ... Cacna1c(12288)

Description

General description

L-type of Voltage-gated Ca2+ Channels (L-type VGCC) is a high voltage-activated channel that exerts spatial and temporal control over cellular calcium concentrations and modulates function such as neurotransmitter release, hormone secretion, muscle contraction, electrical activity, cell metabolism and proliferation, gene expression and neuronal survival. Calcium dependent processes are important in synaptic modification and thus alterations in calcium channel function may be important for both modifying synaptic plasticity and also in age-related neurodegenerative diseases. Calcium channel antagonists are used as antiarrhythmics and in the treatment of hypertension and may even be neuroprotective in Parkinson’s Disease. Calcium Channel α1C subunit is a pore-forming subunit that together with β subunits generates the necessary signal for membrane targeting of class C L-type calcium channels.

Specificity

Anti-Voltage-gated Calcium Channel α1C subunit recognizes the α1C subunit by immunoblotting in rat brain and rat heart membranes. Reactivity has been confirmed for rat, mouse, rabbit and human.

Immunogen

synthetic peptide corresponding to amino acids 848 to 865 of rat α1C.

Application

Anti-Calcium Channel (α1C Subunit) (L-type of Voltage-gated Ca2+ Channel) antibody produced in rabbit has been used in:
• SDS-PAGE
• western blotting
• coimmunoprecipitation

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Rabbit polyclonal anti-Calcium Channel α1C subunit antibody is used to tag class C L-type calcium channel subunit α1C for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as ELISA, immunoblotting, and immunoprecipitation. It is used as a probe to determine the presence and roles of calcium channel subunit α1C in class C L-type voltage-gated Ca2+ channel subunit complexes.

Physical form

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Antihypertensive Agents

Agents that inhibit angiontensin-converting enzymes (ACE) and angiotensin II formation are essential to cardiovascular medicine.1 These inhibitors are used not only to treat essential hypertension an...
Sami Barghshoon
BioFiles v7 n5, 2012, 5–20
Keywords: AGE, Antihypertensives, Cardiovascular, Clinical, Diuretics, PAGE, Reductions

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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