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C1749 Sigma-Aldrich

Monoclonal Anti-Polycystin-1 antibody produced in mouse

clone PKD46, tissue culture supernatant, buffered aqueous solution

Synonym: Anti-PCKD, Anti-PKD1, Anti-Polycystic kidney disease 1 (autosomal dominant), Anti-Polycystic kidney disease-associated protein

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Adhesion Molecules and Related Proteins, Antibodies to Cell and Organelle Proteins,
conjugate   unconjugated
clone   PKD46, monoclonal
biological source   mouse
application(s)   immunohistochemistry: 1:50-1:100 using human kidney sections
species reactivity   rat, human
mol wt   antigen ~500 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   tissue culture supernatant
isotype   IgM
antibody product type   primary antibodies
UniProt accession no.   P98161
Gene Information   human ... PKD1(5310)

Description

General description

Monoclonal Anti-Polycystin-1 (mouse IgM isotype) is derived from the hybridoma PKD46 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human polycystin-1, conjugated to KLH. Polycystin-1 is composed of a large N-terminal extracellular region that contains several ligand-binding domains, multiple transmembrane domains and a cytoplasmic C-tail. It has a wide tissue distribution, with higher expression in the kidney, brain, liver, pancreas, heart and intestine.

Polycystin-1 is a protein encoded by the PKD1 gene in humans. It is also referred as PBP, Pc-1 and TRPP1. Polycystic kidney disease 1 (PKD1) gene is found on chromosome 16. It is a glycoprotein with multiple transmembrane domains and a cytoplasmic C-tail.

Immunogen

synthetic peptide corresponding to amino acids 4282-4300 of human polycystin-1, conjugated to KLH.

Application

Monoclonal Anti-Polycystin-1 antibody produced in mouse has been used in immunoblotting and immunohistochemistry.

Physical form

Solution containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

PKD1 protein is involved in the adhesion of protein-protein and protein-carbohydrate interactions in the extracellular compartment. The most common cause of autosomal dominant polycystic kidney disease (ADPKD) is due to mutation in the PKD1 gene. Alterations in single base substitutions within the coding sequence lead to missense or synonymous mutations in PKD1 gene. ADPKD caused by mutations in PKD1 gene is more severe than that caused by PKD2 gene mutations.

Polycystin-1 is associated with renal tubulogenesis and intracellular signaling pathways.

Safety & Documentation

Safety Information

WGK Germany 
1

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
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References

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