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C1809 Sigma-Aldrich

Collagen from kangaroo tail

Bornstein and Traub Type I (Sigma Type I), powder, suitable for cell culture

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Properties

form   powder
application(s)   cell culture | mammalian: suitable
surface coverage   6‑10 μg/cm2
solubility   aqueous acid: soluble (1 - 2μl acetic acid per ml H2O)
storage temp.   2-8°C

Description

General description

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Application

This collagen has been tested in culture with mammalian cells to verify it is low in endotoxin content. Type I collagen is a component of skin, bone, tendon, and other fibrous connective tissues. It is often used in cell culture as an attachment substratum. Myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have been cultured successfully on films or gels of Type I collagen. Type I collagen differs from other collagens by their low lysine hydroxylation and low carbohydrate composition.

Physical form

Lyophilized from 10 mM glacial acetic acid

Preparation Note

Prepared by a modification of the pepsin extraction method of Niyibizi, et al., J. Biol. Chem., 259, 14170 (1984).

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

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Collagen Usage Recommendations

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Extracellular Matrix Proteins and tools for cell culture optimization

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Overview of 3D Cell Culture: Tools and Techniques

Cell culture techniques are ubiquitous in areas of developmental biology, drug discovery, regenerative medicine and protein production. Since the introduction of cell culture techniques, cells have b...
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What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. It has long been understood to be the structural support for cells since its characteristics set the characteristics...
Keywords: Adhesion, Cell culture, Cellular processes, Epimerizations, Growth factors, Immobilization, Sulfations

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Peer-Reviewed Papers
15

References

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