• USA Home
  • C1926 - Monoclonal Anti-Collagen, Type IV antibody produced in mouse

C1926 Sigma

Monoclonal Anti-Collagen, Type IV antibody produced in mouse

clone COL-94, ascites fluid

Synonym: Collagen Iv Antibody - Monoclonal Anti-Collagen, Type IV antibody produced in mouse - Tested Applications: direct ELISA >= 1:7,000,immunohistochemistry (formalin-fixed, paraffin-embedded sections) protease-digested sections of human tissue 1:500, Collagen Iv Antibody



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells, Antibodies for Mesenchymal Stem Cells,
species reactivity   human
application(s)   dot blot: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested sections of human tissue
  immunohistochemistry (frozen sections): suitable
  indirect ELISA: suitable
  microarray: suitable
clone   COL-94, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... COL4A1(1282), COL4A3(1285), COL4A4(1286), COL4A5(1287), COL4A6(1288)
biological source   mouse
conjugate   unconjugated



human collagen type IV

General description

The composition of the extracellular matrix of all vertebrates is dominated by a class of molecules known as collagens, each with a unique feature suited to its function and location. Type IV collagen is a major constituent of basement membranes. This molecule is a continuous triple helix, about 400 nm in length, composed of at least two different αchain subunits termed α1 (IV) and α2 (IV) which may be composed of the same or different molecules. Upon proteolysis, fragments of 125 kDa, 95 kDa, and 60 kDa are released from a parent α1 (IV) chain, whereas fragments of 115 kDa, 80 kDa, and 45 kDa apparently originate from a distinct α2 (IV) chain. Collagens are known to have multiple functions. They are involved in tissue differentiation, morphogenesis and repair; play a role in the maintenance of tissue architecture; and form the filtration barrier of blood vessels.


Using immunohistochemical staining of basal laminae, the antibody recognizes an epitope located on the α1 and/or α2 chains of human collagen type IV. The antibody shows reactivity with native primate collagen type IV in ELISA and dot blot assay. Using the dot blot technique, the antibody shows no cross-reactivity with human collagen types I, II, III, V, VI and VII, human vitronectin, fibronectin and chondroitin sulfate types A, B and C.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Collagen, Type IV monoclonal antibody may be used in immunoassays such as immunocytochemistry, dot blot and ELISA. The antibody has been used for immunogold staining for electron microscopy, to visualize type IV collagen, in patients with coeliac disease. A minimum working dilution of 1:500 is determined by immunoperoxidase staining of formalin-fixed, paraffinembedded and enzyme-treated human tissue.

Price and Availability

Explore with Confidence

Biomedical Applications

Celebrate Pi Day!
Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?