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  • C2081 - Anti-α-Catenin antibody produced in rabbit

C2081 Sigma-Aldrich

Anti-α-Catenin antibody produced in rabbit

whole antiserum

Synonym: Alpha Catenin Antibody - Anti-α-Catenin antibody produced in rabbit, Alpha Catenin Antibody, Anti-α-Catenin

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Adhesion Molecules and Related Proteins, Antibodies to Cell and Organelle Proteins,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   dot blot: suitable using α-catenin peptide amino acids 890-901 conjugated to BSA
  immunohistochemistry (frozen sections): 1:2,000 using bovine kidney sections
  indirect immunofluorescence: 1:2,000 using cultured MDBK cells
  microarray: suitable
  western blot: 1:4,000 using cultured MDBK cells extract
species reactivity   several mammalian species
mol wt   antigen ~102 kDa
shipped in   dry ice
storage temp.   −20°C
antibody form   whole antiserum
antibody product type   primary antibodies
contains   15 mM sodium azide
Gene Information   human ... CTNNA1(1495)
mouse ... Ctnna1(12385)
rat ... Ctnna1(307505)


General description

Catenin are distinct peripheral cytosolic proteins, α, β, and γ -catenin (102 kDa, 94 kDa and 86 kDa, respectively) are found in varying abundance in many developing and adult tissues. Within its conserved regions, α-catenin shows 30% identity to vinculin, a protein found mainly in focal cell-cell and cell substrate adhesions.


Does not cross-react with β-catenin or γ-catenin (plakoglobin).


synthetic peptide corresponding to the C-terminal region (amino acids 890-901) of human/mouse α-catenin conjugated to KLH.


Anti-α-Catenin antibody has been used:
• in dot blot immunoassay
• in immunoblotting
• in immunofluorescence
• in western blotting
• in immunoprecipitation
• in immunofluorescence staining
• in coimmunoprecipitation


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Catenins bind, directly or indirectly, to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. Cadherins are transmembrane cell surface glycoprotein molecules that mediate calcium-dependent intercellular interactions and are important for tissue morphogenesis. Catenins link E-cadherin to other integral membrane proteins such as Na+ /K+ -ATPase, or to cytoplasmic proteins such as fodrin, ankyrin, sarcoma (Src) and Yes kinases and are modulated by Wnt-1 protooncogene. They are considered good candidates for mediating transduction of cell-cell contact positional signals to the cell interior. α-Catenin appears to be capable of interacting with N-cadherin and P-cadherin. Absence of α-catenin is found in certain tumor cell lines. Frequent reduction of α-catenin levels in human carcinomas of the esophagus, stomach and colon is also reported. Prostate cancer development appears to be correlated with α-catenin gene deletions.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


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Western Blot Protocol | Immunoblotting Protocol

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