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C2206 Sigma-Aldrich

Anti-β-Catenin antibody produced in rabbit

whole antiserum

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Properties

Related Categories AA - CA, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Embryonic Stem Cells,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   dot blot: suitable using the immunogen and related peptides conjugated to BSA
  immunocytochemistry: 1:2,000 using cultured MDBK cells
  immunohistochemistry: 1:2,000 using bovine kidney frozen sections
  western blot: 1:4,000 using cultured MDBK cells
species reactivity   several mammalian species
mol wt   antigen 88-94 kDa
shipped in   dry ice
storage temp.   −20°C
packaging   antibody small pack of 25 μL
antibody form   whole antiserum
Quality Level   200
antibody product type   primary antibodies
contains   15 mM sodium azide
Featured Industry   Research Pathology
Gene Information   human ... CTNNB1(1499)
mouse ... Ctnnb1(12387)
rat ... Ctnnb1(84353)

Description

General description

Catenin are distinct peripheral cytosolic proteins and are classified into α-, β- and γ-catenin (102 kDa, 94 kDa and 86 kDa respectively. It is found in varying abundance in many developing and adult tissues.

Specificity

The antibody does not cross react with α-catenin or γ-catenin (plakoglobin).

Immunogen

synthetic peptide corresponding to the C-terminal region (amino acids 768-781) of human/mouse β-catenin, conjugated with gluteraldehyde to KLH.

Application

Anti-β-Catenin antibody produced in rabbit has been used in
• co-immunoprecipitation
• immunoblotting
• immunofluorescence
• immunoperoxidase assay

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Catenins bind, directly or indirectly, to the conserved cytoplasmic tail domain of the cell adhesion cadherins. The protein, β-catenin, binds directly to the cytoplasmic tail of E-cadherin. Plakoglobin (probably identical to γ-catenin) and β-catenin are structural and possibly functional mammalian homologues of armadillo (arm), a Drosophila protein involved in signal transduction. It binds to the amino terminus of α-catenin and also interacts with the cytosolic protein product of the human tumor suppressor gene Adenomatous polyposis coli (APC). The central core region of β-catenin is involved in mediation of the interaction of cadherin-catenin complex with the epidermal growth factor receptor. The protein, β-catenin, is the target of two signal transduction pathways mediated by the protooncogenes Src and wingless-related integration site (Wnt-1).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Cancer Stem Cells: New Targets for Cancer Therapy

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Keywords: Cancer, Cell attachment, Cell culture, Cell proliferation, Gastrointestinal, Gene expression, Transcription, transformation

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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