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C-22110 Sigma-Aldrich

Endothelial Cell Growth Medium

Kit including Basal Medium and SupplementPack, 500 ml

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Properties

Related Categories Cell Culture, Endothelial Cell Culture, Human Primary Cell Culture, Large Blood Vessels, Media & Supplements,
packaging   pkg of 500 ml
application(s)   cell culture | mammalian: suitable
shipped in   wet ice

Description

General description

Endothelial Cell Growth Medium Kit

Application

Endothelial Cell Growth Medium is a low-serum (2% V/V) medium optimized for the cultivation of endothelial cells from large blood vessels. The media are optimized for primary human cells but can also be used for bovine, porcine, murine, caprine, rabbit, elephant, dog, and rat endothelial cells from large vessels as well as for various endothelial cell lines.Note: For best transfection results we recommend using PromoCell heparin-free Endothelial Cell Growth Medium MV2. Heparin, included all our other our Endothelial Cell Growth Media formulations, may reduce transfection efficiency.

Components

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Quality

All lots of PromoCell Endothelial Cell Media are subjected to comprehensive quality control tests using primary human endothelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested endothelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Preparation Note

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.02 ml / mlEndothelial Cell Growth Supplement: 0.004 ml / mlEpidermal Growth Factor (recombinant human): 0.1 ng / mlBasic Fibroblast Growth Factor (recombinant human): 1 ng / mlHeparin: 90 µg / mlHydrocortisone: 1 µg / ml

Storage and Stability

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Subculture Routine

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Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Endothelial Cell Tube Formation Angiogenesis Assay

The formation of new blood vessels in the body are generated by three processes: vasculogenesis, arteriogenesis and angiogenesis.
Keywords: Angiogenesis, Biochemistry, Cancer, Cell culture, Clinical, Coagulation, Diseases, Growth factors, Infectious Diseases, Metabolism, Microscopy

The Endothelial Cell Assay

Angiogenesis is a tightly regulated cellular event that is balanced by pro- and antiangiogenic signals including integrins, chemokines, angiopoietins, oxygen sensing agents, junctional molecules and ...
Keywords: Adhesion, Angiogenesis, Cancer, Cell culture, Clinical, Diabetes, Drug discovery, Growth factors

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