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C2249 Sigma-Aldrich

Collagen Solution from human fibroblasts

3 mg/mL, sterile-filtered, BioReagent, suitable for cell culture

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Properties

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biological source   human fibroblasts
sterility   sterile-filtered
product line   BioReagent
form   solution
packaging   pkg of 20 mL
concentration   3 mg/mL
surface coverage   6‑10 μg/cm2
suitability   suitable for cell culture
foreign activity   endotoxin ≤5.0 μmol/mg protein
storage temp.   2-8°C

Description

Application

Purified human collagen solution is produced from neo-natal human fibroblasts that have secreted human extracellular matrix (hECM) using a propriety cell culture production system. The human fibroblast cells have undergone significant testing demonstrating safety from viruses and other adventitious agents.

Biochem/physiol Actions

Collagen is an essential ingredient of connective tissue. Studies in a Chinese family show that mutation in COL1A1 (collagenase type I) is linked with type I osteogenesis imperfecta. Collagen is linked with subchondral turnover of bone, and might have potential as marker to determine the state of joint space narrowing and osteophytes in osteoarthritis.

General description

Type I collagen is a major structural component of skin, bone, tendon, and other fibrous connective tissues. It differs from other collagens by its low lysine hydroxylation and low carbohydrate composition. Type I collagen is a heterotrimer composed of two α1(I) chains and one alpha2(I) chain, which spontaneously form a triple helix scaffold at neutral pH and 37 °C.

This product is prepared from the extracellular matrix secreted by normal human fibroblasts and contains a high monomer content. In vitro cultures were prepared using intensively tested human fibroblast cells and purified using a manufacturing process following applicable aspects of cGMP. This process contains built-in, validated steps to insure inactivation of possible prion and/or viral contaminants.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis

Certificate of Origin


Request a Serum Sample

Cell Culture Guide
Protocols & Articles

Articles

Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
Keywords: Adhesion, Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Cell attachment, Cell culture, Cell proliferation, Coagulation, Endocrinology, Growth factors, Hormones

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. It has long been understood to be the structural support for cells since its characteristics set the characteristics...
Keywords: Adhesion, Cell culture, Cellular processes, Epimerizations, Growth factors, Immobilization, Sulfations

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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