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C2249 Sigma-Aldrich

Collagen Solution from human fibroblasts

3 mg/mL, sterile-filtered, BioReagent, suitable for cell culture

  •  NACRES NA.75



Related Categories Attachment Factors, Cell Culture, Reagents and Supplements More...
Quality Level   100
biological source   human fibroblasts
sterility   sterile-filtered
product line   BioReagent
form   solution
packaging   pkg of 20 mL
concentration   3 mg/mL
application(s)   cell culture | mammalian: suitable
surface coverage   6‑10 μg/cm2
foreign activity   endotoxin ≤5.0 μmole/min-mg protein
shipped in   wet ice
storage temp.   2-8°C


General description

Collagen constitutes to one-third of the total protein in humans. Collagen fibril is the basic component of the tissues containing collagen. It is predominantly expressed in the extracellular matrix.

Type I collagen is a major structural component of skin, bone, tendon, and other fibrous connective tissues. It differs from other collagens by its low lysine hydroxylation and low carbohydrate composition. Type I collagen is a heterotrimer composed of two α1(I) chains and one alpha2(I) chain, which spontaneously form a triple helix scaffold at neutral pH and 37 °C.

This product is prepared from the extracellular matrix secreted by normal human fibroblasts and contains a high monomer content. In vitro cultures were prepared using intensively tested human fibroblast cells and purified using a manufacturing process following applicable aspects of cGMP. This process contains built-in, validated steps to insure inactivation of possible prion and/or viral contaminants.


Purified human collagen solution is produced from neo-natal human fibroblasts that have secreted human extracellular matrix (hECM) using a propriety cell culture production system. The human fibroblast cells have undergone significant testing demonstrating safety from viruses and other adventitious agents. Collagen Solution from human fibroblasts has been used as a component of coating solution for human bone marrow chip fabrication. It has also been used in fibroblast-like synoviocytes culturing.

Biochem/physiol Actions

Collagen is an essential ingredient of connective tissue. Studies in a Chinese family show that mutation in COL1A1 (collagenase type I) is linked with type I osteogenesis imperfecta. Collagen is linked with subchondral turnover of bone, and might have potential as marker to determine the state of joint space narrowing and osteophytes in osteoarthritis.

Proteins belonging to the collagen family supports and nourishes body tissues such as tendons, cartilage, skin and sclera of the eye. Collagen type 1 is a versatile building material required for tissue elasticity and maintains its stability and strength. Collagen is also present in byssus thread of invertebrates, which helps in surface attachment. Defective collagen affects the biomechanical property of arterial tissues.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
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What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. It has long been understood to be the structural support for cells since its characteristics set the characteristics...
Keywords: Adhesion, Cell culture, Cellular processes, Epimerizations, Growth factors, Immobilization, Sulfations

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