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C2436 Sigma-Aldrich

Cytochrome c from Saccharomyces cerevisiae

≥85% based on Mol. Wt. 12,588 basis

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Properties

Related Categories Apoptosis and Cell Cycle, Cell Biology, Cell Signaling and Neuroscience, Cytochrome c, Cytochromes,
Quality Level   200
biological source   Saccharomyces cerevisiae
assay   ≥85% based on Mol. Wt. 12,588 basis
form   powder
application(s)   cell analysis: suitable
storage temp.   −20°C
Gene Information   Saccharomyces cerevisiae ... CYC1(853507)

Description

General description

Cytochrome c (Cytc) comprises 104 amino acids and is a nuclear-encoded mitochondrial protein. This peripheral membrane protein is in spherical shape and has a molecular weight of 12,000 Da.

Application

Cytochrome c from Saccharomyces cerevisiae has been used in oocyte injection to induce caspase-3 activation and p38 MAPK (mitogen-activated protein kinase). It has also been used to analyze the aerobic reactions of nitric oxide.

Packaging

25, 100 mg in poly bottle

Biochem/physiol Actions

Cytochrome c (Cytc) participates in mitochondrial electron transport and intrinsic type II apoptosis. It plays a key role in aerobic energy production. This multi-functional enzyme serves as a single electron carrier. Cytc helps in the formation of the apoptosome.

The ready fluctuation of cytochrome c within the cell between ferrous and ferric states, makes it an efficient biological electron-transporter. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal catalyst of respiration, it forms the essential electron-bridge between the respirable substrates and oxygen. Tracking cellular localization of cytochrome c in S. cerervisiae demonstrates that mitochondria are strongly involved in programmed cell death, a process very similar to apoptosis in higher organisms.

Quality

Care has been taken to maintain the native form of the protein. It has not been artificially oxidized or reduced during purification. Contains variable amounts of reduced cytochrome c.

Preparation Note

Prepared without using TCA.

Other Notes

View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enzymeexplorer.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
HA5365000

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO PolyMA-SCX (Ion Exchange)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO PolyMA-SCX (Ion Exchange)
Keywords: Chromatography, High performance liquid chromatography, Separation

HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO Wide Pore C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO Wide Pore C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins on Discovery® BIO PolyMA-SCX (Analyte Set #2)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Discovery® BIO PolyMA-SCX (Analyte Set #2)
Keywords: Chromatography, High performance liquid chromatography

Peer-Reviewed Papers
15

References

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