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  • C2493 - Anti-CENP-A (N-terminal) antibody produced in rabbit

C2493 Sigma

Anti-CENP-A (N-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-CenH3, Anti-Centomeric protein A



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Epigenetics, Antibodies for Gene Regulation and Expression,
species reactivity   human, bovine (predicted)
application(s)   western blot: 0.5-1 μg/mL using HEK-293T cells expressing human CENP-A
clone   polyclonal
concentration   ~1.5 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 17 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CENPA(1058)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to amino acids 4-17 located at the N-terminus of human CENP-A.

General description

Centromere protein A is a protein encoded by the CENPA gene in humans. It is a histone H3 variant that has centromere-specific nucleosomal property and is located at neocentromere found on chromosome 10. CENP-A- binding clusters is associated with nucleosomal blocks within the neocentromeric chromatin.

Physical form

Solution in 0.01 mM phosphate buffered saline, pH 7.4, and 15 mM sodium azide.


Anti-CENP-A (N-terminal) antibody produced in rabbit is suitable for western blotting at a concentration of 0.5-1μg/mL using HEK-293T cells expressing human CENP-A.

Biochem/physiol Actions

The location of the centromere on the chromosome is dependent on the histone H3 variant centromere protein A (CENP-A). The N-terminal tail of CENP-A is highly different from other H3 variants. Serine 7 of CENP-A plays an important role in mitosis-specific phosphorylation. It also plays a key role in kinetochore assembly. Alteration in CENP-A causes various human cancers. CENP-A may play an important role in epithelial ovarian cancer (EOC) and may serve as prognostic marker. It acts as a potential target for gene therapy in the treatment of EOC. The NH2 terminus of human CENP-A is essential for mitotic progression and necessary for the platform function of CENP-A centromeric chromatin in the assembly as well as maintenance of active kinetochores.

Safety & Documentation

Safety Information

WGK Germany  3
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers


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