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C2542 Sigma-Aldrich

Monoclonal Anti-N-Cadherin antibody produced in mouse

clone GC-4, ascites fluid

Synonym: Anti-A-CAM, Anti-A-Cell Adhesion Molecule

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Adhesion, Attachment, and Matrix Factors, Antibodies against Adhesion/Attachment/Matrix Factors, Antibodies for Cell Biology,
conjugate   unconjugated
clone   GC-4, monoclonal
biological source   mouse
application(s)   electron microscopy: suitable
  immunohistochemistry (frozen sections): 1:100 using chicken cardiac muscle
  microarray: suitable
  western blot: 1:100 using chicken or rat cardiac muscle
species reactivity   rabbit, mouse, rat, human, chicken
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
antibody product type   primary antibodies
contains   15 mM sodium azide
Featured Industry   Research Pathology
UniProt accession no.   P19022
Gene Information   human ... CDH2(1000)
mouse ... Cdh2(12558)
rat ... Cdh2(83501)

Description

General description

N-cadherin is a calcium dependent cell adhesion molecule and is present mainly in cancer cells. Monoclonal anti-N-cadherin antibody reacts with the N-terminal portion of the extracellular domain of adipocyte adhesion molecule (ACAM).

Specificity

Monoclonal anti-N-cadherin antibody reacts specifically with A-CAM molecule in human, mouse, rabbit, chicken and rat.

Immunogen

Affinity purified chicken heart A-CAM.

Application

Monoclonal anti-N-cadherin antibody can be used in immunofluorescent microscopy, electron microscopy and immunoblotting to study the receptor localization of intercellular adherens. It may also be used to study the mechanism of adhesion and interaction in tissues.

Monoclonal anti-N-cadherin antibody can be used in immunohistochemistry (1: 100) and as primary antibody in western blotting (1:1000) .

Physical form

Monoclonal Anti-N-Cadherin antibody produced in mouse is supplied as ascites fluid containing 15mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

N-cadherin regulates the Src kinase pathway and also promotes cell motility and invasion. It inhibits adherens junction formation and disrupts existing junctions of cultured lens cells.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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