• USA Home
  • C2562 - Monoclonal Anti-Cytokeratin, pan (Mixture) antibody produced in mouse

C2562 Sigma-Aldrich

Monoclonal Anti-Cytokeratin, pan (Mixture) antibody produced in mouse

clone C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2, ascites fluid

Synonym: Monoclonal Anti-Cytokeratin, pan (mixture), Panck Antibody - Monoclonal Anti-Cytokeratin, pan (Mixture) antibody produced in mouse, Panck Antibody



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytokeratins,
conjugate   unconjugated
clone   C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2, monoclonal
biological source   mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using protease-digested sections of human or animal tissues
  immunohistochemistry (frozen sections): suitable
  indirect immunofluorescence: 1:100 using protease-digested, formalin-fixed, paraffin-embedded sections of human or animal tissues
  western blot: suitable
species reactivity   wide range
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgG1/IgG2a
antibody product type   primary antibodies
contains   7% horse serum and 15 mM sodium azide as preservative


General description

Monoclonal Anti-Cytokeratin, pan (mouse IgG1 and IgG2a isotypes) is a mixture of monoclonal antibodies from the following clones: C-11, PCK-26, CY-90, KS1A3, M20, and A53-B/A2. Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1 (68 kDa), 4 (59 kDa), 5 (58 kDa), 8 (52 kDa), and 6 (56 kDa) are members of the type II neutral-to-basic subfamily expressed in cornified epithelia, non-cornified stratified squamous epithelia, stratified epithelia, simple epithelia and in tissues with natural or pathological turnover respectively. Cytokeratins 10 (56 kDa), 13 (54 kDa, and 18 (45 kDa) are members of the type I acidic subfamily expressed in cornified epithelia, non-cornified stratified squamous epithelia and simple epithelial cells respectively. Cytokeratin peptide 19 (40 kDa) is a type I keratin which can be expressed in both simple and in broad cells of stratifying epithelia at specific sites.


This is a mixture of monoclonal anti-cytokeratin antibodies. It recognizes human cytokeratins 1,4,5,6,8,10,13,18, and 19. It is a broad spectrum reagent, which reacts specifically with a wide variety of normal, reactive, and neoplastic epithelial tissues. The antibody mixture reacts with simple, cornifying and non-cornifying squamous epithelia and pseudostratified epithelia. It does not react with non-epithelial normal human tissues. Increased staining intensity is seen following proteolytic treatment (protease unmasking).


mixture of several monoclonal cytokeratin clones.


Monoclonal Anti-Cytokeratin, pan (Mixture) antibody produced in mouse is suitable for the following applications:
• Immunohistochemistry (formalin-fixed, paraffin-embedded sections) using protease-digested sections of human or animal tissues.
• Immunohistochemistry (frozen sections).
• Indirect immunofluorescence (at a working dilution of 1:100 using protease-digested, formalin-fixed, paraffin-embedded sections of human or animal tissues).
• Immunocytochemical labeling (immunofluorescence ) of cells.
• Western blotting.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Cytokeratins are proteins of keratin-containing intermediate filaments that provide mechanical support and other additional functions in the epithelial cells. It is found in the intracytoplasmic cytoskeleton of epithelial tissue. Epithelial tissue expresses cytokeratin subunits in a specific and stable pattern. Cytokeratins along with vimentin are involved in cell proliferation, migration and differentiation of preodontoblasts and preameloblasts. The intermediate-sized filaments are abundant in human endothelial cells and are mostly of vimentin type.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?