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  • C265 - Monoclonal Anti-CaM Kinase IIα (CaMKIIα) antibody produced in mouse

C265 Sigma

Monoclonal Anti-CaM Kinase IIα (CaMKIIα) antibody produced in mouse

clone 6G9, purified immunoglobulin, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, CAM-CB,
species reactivity   rat
application(s)   indirect immunofluorescence: 1:100 using rat hippocampus
  western blot: 1:2,000
clone   6G9, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt ~50 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   rat ... Camk2a(25400)
biological source   mouse
conjugate   unconjugated



partially purified rat CaM kinase II.

General description

CaM Kinase II α is a Ca2+/calmodulin-dependent kinase that belongs to the serine/threonine protein kinases family. It facilitates the regulation of NMDAR-dependent AMPA receptor trafficking to the synapses. Monoclonal anti-CaM kinase II α (CaMKIIα) antibody can be used for localization of CaM kinase II α subunit (50kD approx.). It can also be used in indirect immunofluorescence (diluted 1: 100). Mouse anti-CaM kinase II α antibody reacts specifically with phosphorylated and non-phosphorylated forms of native and recombinant CaM (Ca2+/calmodulin-dependent) kinase II αsubunit in rats. The product has shown no reactivity for phosphorylated or non-phosphorylated β subunit (60kD).

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Monoclonal anti-CaM kinase II α (CaMKIIα) antibody can be used as a primary antibody in immunohistochemical studies. It can also be used in western blotting (diluted 1:2000).

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  nwg
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Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers


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