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C2730 Sigma-Aldrich

Cellulase from Trichoderma reesei

aqueous solution, ≥700 units/g

Synonym: Celluclast® 1.5L

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Description

General description

Cellulase from Trichoderma reeseiis produced by submerged fermentation. It corresponds to a molecular mass of 68 kDa with optimum pH and temperature of 6 and 52° C, respectively.

Application

Cellulase from Trichoderma reesei has been used:
•  for aqueous extraction of oil from corn germ
•  in the enzymatic hydrolysis of Sweet Sorghum Bagasse (SSB)
•  in the enzymatic digestion of paper grids

Cellulase from Sigma has been used to degrade cello-oligosaccharides into glucose in order to investigate the biodegradability of bioabsorbable bacterial cellulose (BBC).

Packaging

50 mL in glass bottle

Biochem/physiol Actions

Cellulase catalyzes the breakdown of cellulose into glucose. It complexes with β-glucosidase for degrading cellobiose to glucose polymer. The lignin content in straw and furfural residues hampers cellulase production.

Legal Information

A product of Novozyme Corp.

Celluclast is a registered trademark of Novozymes Corp.

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What are the optimal conditions for Product C2730, Cellulase?
For cellulase (C2730) the optimal pH range is 4.5 - 6.0, and the optimal temperature range is 50 - 60°C.
What is the unit definition for the activity of Product C2730, Cellulase?
An EGU (Endoglucanase Unit) is measured relative to a Novozyme cellulase standard. The assay utilizes carboxymethyl cellulose (CMC) as the substrate and the assay is performed at 40°C. at pH 6.0.
What is the protein concentration of Product C2730, Cellulase?
The protein concentration for cellulase (C2730) is not determined.The density of this cellulase solution is approximately 1.2 g/mL.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
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Peer-Reviewed Papers
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References

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