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C2885 Sigma-Aldrich

CHES

≥99.0% (titration)

Synonym: 2-(Cyclohexylamino)ethanesulfonic acid

  • CAS Number 103-47-9

  • Empirical Formula (Hill Notation) C8H17NO3S

  • Molecular Weight 207.29

  •  Beilstein/REAXYS Number 2967601

  •  EC Number 203-115-6

  •  MDL number MFCD00003835

  •  PubChem Substance ID 24892541

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffers A to Z, Good’s Buffers
InChI Key   MKWKNSIESPFAQN-UHFFFAOYSA-N
assay   ≥99.0% (titration)
useful pH range   8.6 - 10.0
pKa (25 °C)   9.3
Featured Industry   Diagnostic Assay Manufacturing

Description

General description

CHES (2-(N-cyclohexylamino)ethanesulphonic acid) is regarded as Good′s buffers for its self-buffering and biocompatible feature. It is used in protein stabilization and does not interact with metals.

Application

CHES has been used:
• as a buffer in mannanase activity assay of antarctic fungal isolates
• as a buffer for DNA digestion and labeling of 2′-deoxyadenosine 3′-monophosphate
• as a component of Wilson and Horne extraction buffer for characterization of rat plasma enzymes

Packaging

1 kg in poly bottle

25, 100, 250 g in poly bottle

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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