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C2900 Sigma-Aldrich

Chloramphenicol Acetyltransferase from Escherichia coli

lyophilized powder, 50,000-150,000 units/mg protein (Lowry)

Synonym: Acetyl-CoA: chloramphenicol 3-O-acetyltransferase, CAT

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Properties

Related Categories 2.3.x.x Acyltransferases, 2.x.x.x Transferases, Application Index, Biochemicals and Reagents, Cell Biology,
form   lyophilized powder
mol wt   75 kDa (three identical subunits)
shipped in   dry ice
storage temp.   −20°C
Gene Information   Escherichia coli ... cat(2847485)

Description

General description

The enzyme has a molecular weight of approximately 78,000 Da and pH optimum of 7.8.

Biochem/physiol Actions

The enzyme responsible for chloramphenicol resistance in bacteria. Catalyzes the conversion of acetyl-CoA + chloramphenicol to CoA + chloramphenicol 3-acetate.

Unit Definition

One unit will convert 1.0 nanomole of chloramphenicol and acetyl-CoA to chloramphenicol 3-acetate and CoA per min at pH 7.8 at 25 °C.

Physical form

Partially purified; contains Tris buffer salts.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Protocols

Enzymatic Assay of Chloramphenicol Acetyltransferase

To standardize a procedure for the determination of the enzymatic assay of Chloramphenicol Acetyltransferase.
Keywords: Enzyme activity, Enzymology, Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers
15

References

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