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C2931 Sigma

Monoclonal Anti-Cytokeratin, pan antibody produced in mouse

clone C-11, ascites fluid

Synonym: Monoclonal Anti-Pan Cytokeratin

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytokeratins,
species reactivity   bovine, frog, rat, human, kangaroo rat, mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using protease-digested sections of human or animal tissues
  immunohistochemistry (frozen sections): suitable
  indirect immunofluorescence: 1:400 using protease-digested, formalin-fixed, paraffin-embedded sections of human or animal tissues
  western blot: suitable
clone   C-11, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
biological source   mouse
conjugate   unconjugated

Description

Immunogen

keratin-enriched preparation from cultured human epidermoid carcinoma cell line A431.

General description

Intermediate-sized filaments are abundant cytoplasmic structural proteins in most vertebrate cells. Cytokeratins, a group of at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 4, 5, 6 and 8 are members of the type II neutral-to-basic subfamily. Cytokeratin peptide 4 (59 kDa) is the secondary type II keratin expressed in noncornified stratified squamous epithelia. Cytokeratin peptide 5 (58 kDa) is the primary type II keratin in stratified epithelia, while cytokeratin type 8 (52 kDa) is a major type II keratin in simple epithelia. Cytokeratin 6 (56 kDa) is a "hyperproliferation" cytokeratin expressed in tissues with natural or pathological high turnover. Cytokeratins 10, 13 and 18 are members of the type I acidic subfamily. Cytokeratin peptide 10 (56 kDa) is the secondary type I keratin expressed in cornified epithelia. Cytokeratin 13 (54 kDa) is the secondary type I keratin expressed in non-cornified stratified squamous epithelia. Cytokeratin 18 (45 kDa) is the primary type I keratin expressed in simple epithelial cells.

Monoclonal Anti-Pan Cytokeratin (clone C-11) recognizes human cytokeratins 4, 5, 6, 8, 10, 13 and 18 in immunoblotting. The antibody reacts with simple, cornifying and non-cornifying squamous epithelia and pseudostratified epithelia. It does not react with non-epithelial normal human tissues. This antibody can be applied to methanol or acetone-fixed, frozen sections, and to formalin-fixed, paraffin-embedded human tissues. Increased staining intensity is seen following proteolytic treatment of formalin fixed tissue. Similarly, methacarn-fixed material is also suitable for cytokeratin demonstration. Monoclonal Anti-Pan cytokeratin exhibits a wide interspecies cross-reactivity (e.g., human, bovine, rat, frog).

Application

Monoclonal anti-cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Mouse monoclonal clone C-11 anti-cytokeratin, pan antibody is a broad spectrum antibody which recognizes an epitope present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. It may aid in the discrimination of carcinomas and non-epithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues, and for determining the origin of poorly differentiated tumors. It is useful for staining of cultured epithelial cell lines. Mouse monoclonal clone C-11 anti-Cytokeratin, pan antibody may be used for the localization of cytokeratins using various immunochemical assays such as immunoblotting, dot blotting and immunohistochemistry (immunofluorescence and immunoenzymatic staining).

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