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  • C2969 - Monoclonal Anti-I-CAM-1 (CD54) antibody produced in mouse

C2969 Sigma-Aldrich

Monoclonal Anti-I-CAM-1 (CD54) antibody produced in mouse

clone 8.4A6, purified immunoglobulin

Synonym: Anti-I-CAM-1, Monoclonal Anti-CD54



Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
conjugate   unconjugated
clone   8.4A6, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
  immunocytochemistry: suitable
  immunohistochemistry (frozen sections): suitable
  indirect ELISA: suitable
species reactivity   human, monkey
mol wt   antigen 85-110 kDa
shipped in   wet ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
UniProt accession no.   P05362
Gene Information   human ... ICAM1(3383)



The antibody recognizes the human CD54 (I-CAM-1) surface antigen by flow cytometry, ELISA, immunocytochemistry, and immunohistochemistry. It inhibits human leukocyte adhesion to activated endothelial cells and stimulates homotypic aggregation of SKW3 cells. The epitope recognized by the antibody is localized in domain D2 of the CD54 molecule.
5th Workshop: code no. S 100


TNF-α activated human endothelial cells.

Target description

CD54 (I-CAM-1) is a glycoprotein involved in mediation of intercellular adhesion. CD54 is expressed by activated endothelial cells and is also detected on cells of many other lineages and in plasma.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Preparation Note

Product filtered through a 0.2 μm filter

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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