• USA Home
  • C3104 - Anti-CD30 antibody produced in goat

C3104 Sigma-Aldrich

Anti-CD30 antibody produced in goat

affinity isolated antibody, lyophilized powder



Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology,
conjugate   unconjugated
clone   polyclonal
biological source   goat
application(s)   indirect ELISA: 0.5-1.0 μg/mL
  western blot: 0.1-0.2 μg/mL
species reactivity   mouse
form   lyophilized powder
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   Q60846
Gene Information   mouse ... Tnfrsf8(21941)


General description

CD30 is a type I transmembrane glycoprotein of the TNF receptor superfamily. The extracellular domain of recombinant mouse CD30/Fc is fused to the carboxyterminal 6X histidine-tagged Fc portion of human IgG1 by a polypeptide linker.


Shows no cross-reactivity with recombinant human CD30. The antibody will stimulate the bioactivity of mouse splenic B cell proliferation in the presence of IL-4 and IL-5.


recombinant mouse CD30, extracellular domain, expressed in NSO cells.


Anti-CD30 is suitable for western blotting at a concentration of 0.1-0.2μg/mL and indirect ELISA at a concentration of 0.5-1.0μg/mL

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline


Reconstitute with 1 mL sterile PBS to yield 0.1 mg/ml solution.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Anti-CD30 recognizes recombinant mouse CD30 by immunoblotting and ELISA. In immunoblotting and ELISA, this antibody does not cross-react with recombinant human CD30. Reduced mouse CD30/Fc monomer has a calculated molecular mass of approximately 55 kDa. Due to glycosylation, the recombinant protein migrates as an approximately 90 -100 kDa protein in SDS-PAGE under reducing conditions. CD30 specific IgG is purified from goat serum using mouse CD30 affinity chromatography. CD30 has a critical role in the pathophysiology of Hodgkin′s disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. Anti-CD30 is developed in goat using purified recombinant mouse CD30 extracellular domain, expressed in mouse NSO cells as immunogen.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?