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C3172 Sigma-Aldrich

Creatininase from microorganisms

lyophilized powder, 100-300 units/mg protein

Synonym: Creatinine Amidohydrolase



Related Categories 3.5.x.x Acting on C-N other than peptides, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Biology,
form   lyophilized powder
mol wt   ~175 kDa
composition   Protein, 65-85%
foreign activity   Creatinase and urease ≤1%
  Hexokinase and ATPase ≤0.1%
storage temp.   2-8°C



Creatininase from microorganisms may be used in the preparation of amperometric biosensor by co-immobilization with other enzymes for the determination of creatinine.

This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.


1000 units in glass bottle

Biochem/physiol Actions

Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the β-strands and the N termini of the main α-helices. These zinc ions indicate the location of the active site.

Physical properties

Isoelectric point: 4.7
Michaelis constants: 3.2 x 10‾2M (Creatinine), 5.7 x 10‾2M (Creatine)
Structure: 6 subunits per mol of enzyme (One mol of zinc is bound to each subunit)
Inhibitors: Ag+, Hg++, N-bromosuccinimide, EDTA
Optimum pH: 6.5 − 7.5
Optimum temp: 70°C
pH Stability: pH 7.5 − 9.0 (5°C, 16hr)
Thermal stability: Below 70°C (pH 7.5, 30 min)

Unit Definition

One unit will hydrolyze 1.0 mmole of creatinine to creatine per min at pH 8.0 and 25 °C

Physical form

Lyophilized powder containing sucrose and BSA as stabilizers

Analysis Note

Protein determined by biuret.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis


Certificate of Origin

Protocols & Articles


Assay Procedure for Creatininase

The appearance of creatinine-picrate (orange dye based on Jaffe's reaction) is measured at 520nm by spectrophotometry.
Keywords: Extinction coefficient, Sample preparations

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers


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