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C3237 Sigma-Aldrich

Anti-Caveolin-1 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-Caveolin-1, Anti-VIP21

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Caveolae, Antibodies to Cell Membrane,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   indirect immunofluorescence: 1:500 using mouse fibroblast NIH3T3 cell line
  microarray: suitable
  western blot: 1:2,000 using whole cell extract of the human umbilical vein endothelial HUVEC cell line and the rat fibroblast Rat-1 cell line
species reactivity   rat, human, mouse
mol wt   antigen 22 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   Q03135
Gene Information   human ... CAV1(857)
mouse ... Cav1(12389)
rat ... Cav1(25404)

Description

General description

Anti-Caveolin-1 is developed in rabbit using a synthetic peptide corresponding to a region at the N-terminus of human caveolin-1 conjugated to keyhole limpet hemocyanin (KLH) as immunogen. Caveolin-1 (CAV1) is a multifunctional scaffolding protein, that has three exons. It is located on chromosome 7q31.

Caveolin, a 20-24-kDa integral transmembrane protein, has been identified as a principal component of caveolae membranes. Caveolin (also termed VIP21) exists in several isoforms termed caveolin-1, caveolin-2 and caveolin-3. Caveolin-1 (20-22 kDa) can exist as two isoforms, caveolin-1α and -1β due to alternative splicing of the mRNA.

Specificity

The sequence is highly conserved in many species, e.g., identical in rat, mouse, bovine, and dog caveolin-1. The sequence is not found in caveolin-2 or caveolin-3.

Immunogen

synthetic peptide corresponding to a region at the N-terminus of human caveolin-1 (amino acids 2-20).

Application

Anti-Caveolin-1 antibody produced in rabbit has been used in:
• indirect immunofluorescence
• in confocal microscopy
• western blotting

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Caveolin family members function as scaffolding proteins to organize and concentrate specific lipids such as cholesterol and glycosylphosphatidylinositol (GPI) and lipid modified signaling molecules within caveolae membranes. Staining of caveolin-1 in immunoblotting is specifically inhibited with caveolin-1 immunizing peptide (human, amino acids 2-20).†

Caveolin-1 is expected to play a major role in the growth and evolution of cancer. It participates in glucose metabolism. It may also participate in angiogenesis. Cav-1 helps to control the expression of glucose transporters.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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Add to Cart

C4490 Anti-Caveolin-1 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
C9992 Anti-Caveolin-2 antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution, suitable for western blot: 1 μg/mL

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