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C3306 Sigma-Aldrich

Calcium chloride dihydrate

for molecular biology, ≥99.0%

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Properties

Related Categories Biochemicals, Buffer / Buffer Salts, Buffers / Buffer Salts, Buffers / Buffer salts, Calcium,
Quality Level   200
grade   for molecular biology
assay   ≥99.0%
mp   176 °C (dec.) (lit.)
solubility   H2O: 294 mg/mL, clear, colorless
foreign activity   DNase, RNase, protease, none detected
SMILES string   O.O.Cl[Ca]Cl
InChI   1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2
InChI key   LLSDKQJKOVVTOJ-UHFFFAOYSA-L

Description

Application

Calcium chloride dihydrate has been used:
to prepare competent E. coli for transformation(118)
in the preparation of calcium polyphosphate nanospheres and to induce alkaline phosphatase(119)
as a component of renaturation buffer for the zymogram of Staphylococcus aureus crude enzyme extracts(120)

Packaging

100, 250, 500 g in poly bottle

Biochem/physiol Actions

Calcium chloride is used in the heat-shock transformation of microbes with the plasmid. The positive charged calcium forms a cation bridge with phosphate of DNA and negative charged phosphorylated lipid A in lipopolysaccharide (LPS) aiding the DNA transformation into the microbes.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
RTECS 
EV9810000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles
Peer-Reviewed Papers
15

References

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