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C3389 Sigma-Aldrich

Acetylcholinesterase from Electrophorus electricus (electric eel)

Type VI-S, lyophilized powder, 200-1,000 units/mg protein

Synonym: AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Acetylcholinesterase (AChE), True, RBC or Erythrocyte Cholinesterase, E.C. 3.1.1.7, Analytical and Industrial Enzymes, Application Index,
Quality Level   200
type   Type VI-S
form   lyophilized powder
specific activity   200-1,000 units/mg protein
mol wt   280 kDa
composition   Protein, ≥60% biuret
solubility   20 mM Tris HCl buffer, pH 7.5: soluble 1.0 mg/mL, clear(lit.)
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   −20°C

Description

General description

Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)

Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.

Application

The enzyme from sigma has been used as a reference to to evaluate the effect of aspartame metabolites on hippocampal acetylcholinesterase activity. The enzyme has also been used in immobilization studies for the rapid detection of acetylthiocholine chloride.

Packaging

10000 units in poly bottle

500, 2000 units in glass bottle

Biochem/physiol Actions

Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.

Unit Definition

One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.

Physical form

Lyophilized powder containing Tris buffer salts

Preparation Note

This enzyme dissolves in 20 mM Tris-HCl buffer, pH 7.5 at 1 mg/mL concentration, yielding a clear solution.

Analysis Note

The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

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