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  • C3470 - Anti-Connexin-32 (265-279) antibody produced in rabbit

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C3470 Sigma-Aldrich

Anti-Connexin-32 (265-279) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Adhesion Molecules and Related Proteins, Antibodies to Cell and Organelle Proteins,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry (frozen sections): 1:600 using rat liver tissue
  microarray: suitable
  western blot: 1:600 using a rat liver membrane preparation
species reactivity   mouse, rat, human
mol wt   antigen 27 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   P08034
Gene Information   human ... GJB1(2705)
mouse ... Gjb1(14618)
rat ... Gjb1(29584)

Description

General description

Connexins (Cx) are a multi-gene family of highly related proteins with molecular weights ranging from 26 to 70 kD. The structure of connexin molecules includes a cytoplasmic N-terminal region, four transmembrane domains, two extracellular loops, and a C-terminal cytoplasmic tail of varying length. Anti-Connexin 32 is developed in rabbit using synthetic peptide Lys- Arg- Ser-Pro- Gly- Thr- Gly- Ala-Gly- Leu- Ala- Glu- Lys-Ser- Asp- Arg conjugated to KLH with glutaraldehyde as immunogen.

Gap junction protein, connexin-32 (Cx32) is encoded by gap junction protein β 1 (GJB1) gene, localized on the human chromosome X. This gene is expressed in the central nervous system (CNS) in oligodendrocytes and other neuronal populations.

Immunogen

synthetic human/rat connexin-32 peptide (amino acids 265-279 with an N-terminally added lysine).

Application

Anti-Connexin-32 (265-279) antibody produced in rabbit has been used in western blot analysis and immunocytochemistry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Mutations of the gap junction protein β 1 (GJB1) gene results in the X-linked form of Charcot-Marie-tooth disease (CMTX1). Cx32 participates in the liver regeneration after partial hepatectomy.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Carcinogenesis and Epigenetics

Cancer research has revealed that the classical model of carcinogenesis, a three step process consisting of initiation, promotion, and progression, is not complete. The expansion of the carcinogenesi...
Vicki Caligur
BioFiles 2008, 3.5, 18.
Keywords: Acetylations, Adhesion, Alkylations, Apoptosis, Biofiles, Cancer, Carcinogens, Cell division, Cell proliferation, Cell signaling, DNA microarrays, Drug discovery, Environmental, Epigenetics, Events, Gene expression, Genetic, Hormones, Metabolism, Methylations, Microarray Analysis, Mutagens, PAGE, Recombination, Reductions, Sequences, Transcription, Type, transformation

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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