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C3710 Sigma-Aldrich

Ciliary Neurotrophic Factor human

CNTF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym: CNTF

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Description

General description

The CNTF (ciliary neurotrophic factor) gene encodes a cytokine, and is mapped to human chromosome 11q12.1.

Application

Ciliary Neurotrophic Factor human has been used to study its effect on photoreceptor neuroprotection and Muller glial cell proliferation in zebrafish retina. It has also been used to study the effects of intravitreal injection of ciliary neurotrophic factor (CNTF) on photoreceptor injury and BRB (blood-retinal barrier) breakdown in transgenic model.

Biochem/physiol Actions

Ciliary neurotrophic factor (CNTF) was first identified as a survival factor for neurons from the ciliary ganglion of chicken embryos. Most of its known actions are restricted to cells of the nervous system, including motor neurons, sympathetic ganglion neurons, sensory neurons, hippocampal neurons, and medial septal neurons. CNTF also prevents degeneration of motor axons after axotomy and promotes astrocyte differentiation and oligodendrocyte survival and maturation. Outside the nervous system, CNTF maintains embryonic stem cells in an undifferentiated, pluripotent state. CNTF is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM). CNTF exerts its actions through the activation of the high-affinity CTNF receptor complex, which contains the ligand-binding α-subunit (CNTF Rα) and two signal transducing β-subunits (LIF Rβ and gp130). The LIF Rβ subunit is also shared by receptors for LIF and OSM. The gp130 subunit is also shared by receptors for LIF, OSM, IL-6, and IL-11. CNTF is localized in the cell nucleus subsequent to receptor binding. Human and rat CNTF share ~83% sequence homology and show cross-reactivity in bioactivity.

Physical form

lyophilized from 10 mM citric acid, pH 3.0, containing 75 mM NaCl + 0.5-1.0 mg BSA

Reconstitution

reconstitute with water to 0.1-1.0 mg/ml

Analysis Note

The proliferative activity is tested in a cell proliferation assay using the cytokine-dependent human erythroleukemic cell line, TF-1.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Neurotrophic Factors

Neurotrophic factors are agents that are important for survival, growth, or differentiation of discrete neuronal populations. Based on amino acid sequence homologies of cytokines and receptors and ba...
Jennifer Fries
BioFiles 2009, 4.5, 19.
Keywords: Cell proliferation, Dopamine agents, Growth factors

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Peer-Reviewed Papers
15

References

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