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C3865 Sigma-Aldrich

Monoclonal Anti-N-Cadherin antibody produced in mouse

clone GC-4, purified from hybridoma cell culture

Synonym: Anti-A-CAM, Anti-A-Cell Adhesion Molecule

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Adhesion, Attachment, and Matrix Factors, Antibodies against Adhesion/Attachment/Matrix Factors, Antibodies for Cardiac Stem Cells,
conjugate   unconjugated
clone   GC-4, monoclonal
biological source   mouse
application(s)   electron microscopy: suitable
  flow cytometry: suitable
  immunocytochemistry: suitable
  immunohistochemistry: 10-20 μg/mL using rat cardiac muscle frozen sections
  western blot: 10-20 μg/mL using chicken cardiac muscle or COS-7 cell extracts
species reactivity   rat, chicken, monkey, mouse, rabbit, human
mol wt   antigen 135 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
Featured Industry   Research Pathology
UniProt accession no.   P19022
Gene Information   human ... CDH2(1000)
mouse ... Cdh2(12558)
rat ... Cdh2(83501)

Description

General description

N-Cadherin is a member of the cadherin family of proteins that mediate cell-cell adhesion in a calcium dependent manner. N-cadherin is a transmembrane glycoprotein that localizes in the cell-cell adherens junction on the plasma membrane. The protein is known to be localized in the cell membrane.

Application

Anti-N-Cadherin antibody is suitable for the following procedures:
• electron microscopy
• flow cytometry
• immunocytochemistry
• immunohistochemistry at a concentration of 10-20μg/mL using rat cardiac muscle frozen sections
• western blot at a concentration of 10-20μg/mL using chicken cardiac muscle or COS-7 cell extracts

Physical form

0.2 μm-filtered solution in 0.01 M phosphate buffered saline, pH 7.4.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Packaging

200 μL in glass bottle

Biochem/physiol Actions

CDH11 gene encodes a type II classical cadherin that mediate calcium-dependent cell-cell adhesion.

Higher expression of CDH2 gene was observed in tumor cells. It is known to induce the invasive and chemoresistant attributes to the cancer cells. N-glycosylation of N-cadherin is associated with the process of tumorigenesis especially in mammary gland, renal system, and ovary. Loss of expression of the gene was observed in adrenocortical carcinoma.

Other Notes

Clone GC-4 is also known as GB-9.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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