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C3921 Sigma-Aldrich

Creatinase from Pseudomonas sp.

recombinant, expressed in E. coli, lyophilized powder, 10-15 units/mg protein

Synonym: Creatine Amidinohydrolase

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Properties

Related Categories 3.5.x.x Acting on C-N other than peptides, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Biology,
Quality Level   200
recombinant   expressed in E. coli
form   lyophilized powder
specific activity   10-15 units/mg protein
mol wt   45 kDa
composition   Protein, ~70% biuret
storage temp.   2-8°C

Description

Application

Creatine amidinohydrolase is a clinically important enzyme used in the determination of creatinine in blood and urine.

Packaging

500 units in poly bottle

Biochem/physiol Actions

Creatinase is a homodimer with subunit molecular weights of approximately 45 kDa. It catalyzes the hydrolysis of creatine, in the presence of H2O, to form urea and sarcosine.

Unit Definition

One unit will hydrolyze 1.0 μmole of creatine to urea and sarcosine per min at pH 7.5 at 37 °C.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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