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C3989 Sigma-Aldrich

Anti-Calpain-2, Large Subunit antibody produced in rabbit

1 mg/mL, affinity isolated antibody, buffered aqueous glycerol solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Protein Degradation, Antibodies to Proteolysis/Proteases,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 1:1,000
species reactivity   rat, mouse, human
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   1 mg/mL
UniProt accession no.   P17655
Gene Information   human ... CAPN2(824)
mouse ... Capn2(12334)
rat ... Capn2(29154)

Description

General description

Calpains are calcium-dependent cysteine proteases that are expressed ubiquitously in eukaryotes. Calpains regulate diverse cellular processes such as development, apoptosis and signal transduction . Calpain-2 has been implicated in the development of synaptic plasticity and cataract . Anti-Calpain-2 is specific for the large subunit of calpain-2 in humans, mice and rats. The antibody does not bind to other members of the calpain protein family.

Immunogen

synthetic peptide corresponding to the large subunit of human calpain II (m-calpain, Clpn02, calpain-2).

Application

Anti-Calpain-2, Large Subunit antibody is suitable for use in western blot (1:1000) and immunoblot (80kDa and 58kDa).

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Physical form

Solution in phosphate buffered saline containing 50% glycerol, 1% bovine serum albumin, and 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

WGK Germany 
1

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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