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C4063 Sigma-Aldrich

C Reactive Protein from human fluids

buffered aqueous solution

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Properties

Related Categories Biochemicals and Reagents, Cell Biology, Cell Signaling and Neuroscience, Immune Cell Signaling and Blood, Immune System Regulation,
Quality Level   200
biological source   human fluids
assay   ≥90% (SDS-GE)
form   buffered aqueous solution
application(s)   cell analysis: suitable
storage temp.   2-8°C
Gene Information   human ... CRP(1401)

Description

General description

C Reactive Protein (CRP) is a homopentameric protein, which belongs to the pentraxin family. It is produced in liver hepatocytes, smooth muscle cells, macrophages and adipocytes. CRP gene is located on human chromosome 1q23.2.

Application

C Reactive Protein from human fluids has been used:
• to investigate the neutrophil extracellular traps (NETs)-dependent generation of monomeric CRP (mCRP) from pentameric CRP (pCRP)
• to evaluate its effects on areas at risk (AAR) of myocardium with ischemia-reperfusion injury
• to measure intracellular levels of reactive oxygen species (ROS)

Biochem/physiol Actions

C Reactive Protein (CRP) plays a critical role in apoptosis, phagocytosis, nitric oxide (NO) release and the secretion of cytokines, such as interleukin-6 and tumor necrosis factor-α. CRP acts as a marker for cardiovascular diseases, rheumatoid arthritis and cancer.
C reactive protein (CRP) is an acute phase protein. Serum levels in patients with atherosclerosis is predictive of increased risk of myocardial infarction (MI) and stroke. The cytokine IL-6 is thought to be the key mediator in hepatocyte secretion of acute phase proteins including CRP. CRP mediates innate immunity by binding to microbial polysaccharides and to ligands exposed on damaged cells. The binding activates the classical complement pathway (C1, C4, C2, C3 but not C5-9). Opsonization of the substrates leads to their uptake by phagocytic cells and limits the inflammatory response.

Packaging

Package size based on protein content.

Other Notes

CRP is isolated from human fluids (Ascitic/Pleural).

Physical form

Solution in 0.02 M Tris, 0.28 M sodium chloride, 0.005 M calcium chloride, pH 7.8 - 8.2, containing 0.1% sodium azide.

Analysis Note

Protein determined by Lowry.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Plasma Derived Proteins and Enzymes

Despite their complexity, blood and plasma are abundant biological resources for the discovery of drug targets and biomarkers for human disease. It is estimated that plasma may contain as many as 40,...
BioFiles 2006, 1.5, 2.
Keywords: Applications, Gas chromatography, Type

Peer-Reviewed Papers
15

References

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