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C4129 Sigma-Aldrich

α-Chymotrypsin from bovine pancreas

Type II, lyophilized powder, ≥40 units/mg protein

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Properties

Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Chymotrypsin, alpha-,
Quality Level   300
type   Type II
form   lyophilized powder
mol wt   25 kDa
composition   protein, ≥85%
color   white to off-white
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   −20°C
Gene Information   cow ... CTRB1(618826)

Description

Application

The enzyme from Sigma has been used to study the structure-function relationship in glycosylated α-chymotrypsin using immobilized metal-ion affinity chromatography (IMAC) and immobilized metal-ion affinity capillary electrophoresis (IMACE).

Packaging

1, 10 g in poly bottle

250, 500 mg in poly bottle

Biochem/physiol Actions

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu2+ and Hg2+.

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

Unit Definition

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

Preparation Note

Produced from 3× crystallized chymotrypsinogen

Analysis Note

Protein determined by E1%/280

Other Notes

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
GC3050000

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the molecular weight of alpha-chymotrypsin from bovine pancreas, product C4129?
The molecular weight of alpha chymotrypsin, reported in the literature, is approximately 25 kDa.
How do you recommend preparing a solution of Product No. C4129?
It is recommended to reconstitute Product No. C4129 in 1 mM hydrochloric acid containing 2 mM calcium chloride. The calcium functions as a stabilizer, and possibly an activator, of the enzyme.
How can solutions of alpha chymotrypsin (Product No. C4129) be stored?
Stock solutions prepared in 1 mM HCl, containing 2 mM calcium chloride, can be stored at -20 ° for about one week.
What is the pH optimum for the enzyme, Product No. C4129?
The pH optimum for alpha-chymotrypsin is between pH 7.5-8.5. Please see the following reference: Wilcox, P.E., Chymotrypsingogens-chymotrypsins, Methods in Enzymology, 19, 64-80 (1970).
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Protocols & Articles

Articles

HPLC Analysis of Proteins on Discovery® BIO PolyMA-SCX (Analyte Set #2)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Discovery® BIO PolyMA-SCX (Analyte Set #2)
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins on TSKgel® Porous (Phenyl-5PW and Ether-5PW) and Non-Porous (Butyl-NPR) HIC Columns

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on TSKgel® Porous (Phenyl-5PW and Ether-5PW) and Non-Porous (Butyl-NPR) HIC Columns
Keywords: Chromatography, High performance liquid chromatography

Protocols

Procedure for Enzymatic Assay of Alpha Chymotrypsin (EC 3.4.21.1)

This procedure may be used for the determination of α‑Chymotrypsin activity using N-Benzoyl-L-tyrosine ethyl ester (BTEE) as the substrate. It is not to be used to assay α‑Chymotrypsin agarose (Catal...
Keywords: Extinction coefficient

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Peer-Reviewed Papers
15

References

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