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C4140 Sigma

Citrate Synthase from pigeon breast muscle

ammonium sulfate suspension, 80-150 units/mg protein (modified Warburg-Christian)

Synonym: Citrate condensing enzyme, Citrate oxaloacetate lyase (CoA-acetylating)



Related Categories 4.1.x.x C-C bonds, 4.x.x.x Lyases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
form   ammonium sulfate suspension
foreign activity   isocitrate dehydrogenase and aconitase ≤0.01%
  malic dehydrogenase ≤0.1%
storage temp.   2-8°C


Unit Definition

One unit will form 1.0 μmole of citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37 °C.

Physical form

Crystalline suspension in 2.2 M (NH4)2SO4 solution, pH 7.0, containing 6 mM phosphate and 0.5 mM citrate

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Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

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C3260 Citrate Synthase from porcine heart, ammonium sulfate suspension, ≥100 units/mg protein

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