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  • C4231 - Anti-phospho-β-Catenin (pSer33/pSer37) antibody, Mouse monoclonal

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C4231 Sigma-Aldrich

Anti-phospho-β-Catenin (pSer33/pSer37) antibody, Mouse monoclonal

clone BC-22, purified from hybridoma cell culture

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Embryonic Stem Cells, Antibodies for Epigenetics and Nuclear Signaling,
conjugate   unconjugated
clone   BC-22, monoclonal
biological source   mouse
application(s)   indirect ELISA: suitable
  microarray: suitable
  western blot: 5-10 μg/mL using whole cell extract of cultured human 293 cells treated with proteasome inhibitor MG132
species reactivity   human, rat, mouse, chicken
mol wt   antigen ~94 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2b
antibody product type   primary antibodies
concentration   ~2 mg/mL
UniProt accession no.   P35222
Gene Information   human ... CTNNB1(1499)
mouse ... Ctnnb1(12387)
rat ... Ctnnb1(84353)

Description

General description

CTNNB1 (catenin β 1) gene is mapped to human chromosome 3p21. The encoded protein β-catenin is localized to the nucleus.

Specificity

The antibody is inhibited more efficiently by the pSer37 peptide than by the pSer33 peptide and is not inhibited by the nonphos­phorylated peptide. It does not detect the unphos­phorylated or the Ser33 phosphorylated protein and does not recognize phosphorylated plakoglobin.

Immunogen

synthetic phosphopeptide corresponding to the region that contains serines 33 and 37 of human β-catenin.

Application

Monoclonal Anti-phospho-ß-Catenin (pSer33/pSer37) antibody is suitable for:
• indirect ELISA
• microarray
• western blot at a dilution of 5-10μg/mL using whole cell extract of cultured human 293 cells treated with proteasome inhibitor MG132.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

β-catenin is mapped on the short arm of chromosome 3. It is useful for differential diagnosis of Osteoblastoma (OB) and osteosarcoma (OS). β-catenin signaling pathway may contribute to cholangiocarcinoma (CCA) cell proliferation. It may serve as a prognostic marker for CCA progression and hence can be significant in the CCA therapy target.
In Wnt/β-catenin signal pathway β-Catenin participates as a signal transduction molecule. The pathway is associated with development, tissue homeostasis and oncogenic transcription. Upregulation of the gene is associated with glioma cell proliferation. The protein encoded has dual function that regulates the coordination of cell-cell adhesion by linking cytoskeleton and cellular junctions and gene transcription. Wnt/β-catenin signal pathway is known to induce progression colon cancer progression.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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