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  • C4851 - Anti-Caspase 1 (ICE) antibody produced in rabbit

C4851 Sigma

Anti-Caspase 1 (ICE) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-ICE



Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Caspases,
species reactivity   rat, human, mouse
application(s)   immunocytochemistry: 10 μg/mL using human HL-60 cells
  western blot: 0.5-2 μg/mL using human HL-60 cell lysate
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 20 kDa (p20 subunit)
  antigen mol wt 45 kDa (p45 proenzyme)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CASP1(834)
mouse ... Casp1(12362)
rat ... Casp1(25166)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to amino acid residues 129-152 of human caspase 1 conjugated to KLH.

Physical form

Solution of antiserum in 0.1 M Tris-glycine, pH 7.4, containing 0.15 M sodium chloride, and 0.05% sodium azide.


Recognizes the p20 subunit of reduced human caspase 1 isoforms and the p45 proenzyme.


Anti-Caspase 1 (ICE) antibody produced in rabbit has been used in immunoblot assay to analyse CASP-3 and -7 cleavage of WT and DKO cells treated with etoposide. It is suitable for immunocytochemistry at a concentration of 10μg/mL using human HL-60 cells. It is also suitable for western blot at a concentration of 0.5-2μg/mL using human HL-60 cell lysate.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

ICE1 is a MYC-like basic helix-loop-helix transcription factor. It is an upstream activator of the cold-induced transcriptome. ICE1 belongs to the bHLH subfamily 9. It specifically binds to the MYC recognition sites (5′-CANNTG-3′) on the CBF3/DREB1A promoter. ICE1 regulates the cold-induced transcription of CBF/DREB1 genes.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  nwg
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers


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