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  • C4951 - Cholesterol-Water Soluble

C4951 Sigma-Aldrich

Cholesterol-Water Soluble

powder, BioReagent, suitable for cell culture

Synonym: Cholesterol–methyl-β-cyclodextrin

  •  NACRES NA.75



Related Categories Cell Culture, Lipids and Lipid Carriers, Reagents and Supplements, Solubilizing Agents
Quality Level   200
biological source   ovine wool
product line   BioReagent
form   powder
application(s)   cell culture | mammalian: suitable
solubility   H2O: 300 mg/mL
shipped in   ambient
storage temp.   −20°C



Cholesterol-Water Soluble has been used:
• in free cholesterol (FC) overloading of rat smooth muscle cells (SMCs)
• in cytotoxicity assay
• for the incubation of CD4+ T cells with Helicobacter pylori
• to study the membrane cholesterol level in SH-SY5Y human neuroblastoma cells
• to study the effects of cholesterol on cell viability and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction in primary cultured rat hippocampal neurons
• as a supplement for growth of bacterial culture
• to examine the interaction of S protein with angiotensin-converting enzyme 2 (ACE2)
• to investigate the effects of cholesterol on potassium currents in the inner hair cells of guinea pigs
• for reversible inhibition of proteinase K


30 mg in poly bottle

Package size based on cholesterol

Other Notes

Contains approx. 40 mg of cholesterol per gram; balance methyl-β-cyclodextrin.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How much cholesterol will I be getting in the 30 mg package of Product C4951, Cholesterol-Water Soluble?
The package size is based on the amount of cholesterol present.There is approximately 40 mg of cholesterol per gram of material, the balance being Methyl-β-cyclodextrin.
What is the molecular weight of Product C4951, Cholesterol-Water Soluble?
386.65 g/mol
How do I calculate the molarity of Product C4951, Cholesterol-Water Soluble?
Calculate the molarity based on the cholesterol content of your solution.For instance, if you dissolve 10 mg of material in water, approximately 0.4 mg of that 10 mg is cholesterol.
What concentration of Product C4951, Cholesterol-Water Soluble, is used in the cell culture test?
The water soluble cholesterol is tested for cell culture use at approximately 7.6 micrograms per/L.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
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