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C4951 Sigma

Cholesterol-Water Soluble

powder, BioReagent, suitable for cell culture

Synonym: Cholesterol–methyl-β-cyclodextrin



Related Categories Cell Culture, Lipids and Lipid Carriers, Reagents and Supplements, Solubilizing Agents
product line   BioReagent
form   powder
solubility   H2O: 300 mg/mL
suitability   suitable for cell culture
storage temp.   −20°C


Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Other Notes

Contains approx. 40 mg of cholesterol per gram; balance methyl-β-cyclodextrin.


30 mg in poly bottle

Package size based on cholesterol

Price and Availability

Milli-Q® Water Purification Solutions
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What is the cholesterol content of Product C4951,Cholesterol-Water Soluble?
You can find the lot specific value for the cholesterol content on the Certificate of Analysis.  Certificates of Analysis can be viewed on our website by entering the lot number on the product detail page (for this product):  http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/C4951
How much cholesterol will I be getting in the 30 mg package of Product C4951, Cholesterol-Water Soluble?
The package size is based on the amount of cholesterol present.  There is approximately 40 mg of cholesterol per gram of material, the balance being Methyl-ß-cyclodextrin.
What is the molecular weight of Product C4951, Cholesterol-Water Soluble?
386.65 g/mol
How do I calculate the molarity of Product C4951, Cholesterol-Water Soluble?
Calculate the molarity based on the cholesterol content of your solution.  For instance, if you dissolve 10 mg of material in water, approximately 0.4 mg of that 10 mg is cholesterol.
How should I dissolve Product C4951, Cholesterol-Water Soluble?
To ensure optimal solubility, it is suggested that stock solutions >50 mg/mL (and preferably >100 mg/mL) be prepared, and then diluted to the desired concentration. If a final cholesterol concentration is desired which results in poor cholesterol solubility, addition of uncomplexed cyclodextrin (Sigma product C0926) helps.
What concentration of Product C4951, Cholesterol-Water Soluble, is used in the cell culture test?
The water soluble cholesterol is tested for cell culture use at approximately 7.6 micrograms per/L.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
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Peer-Reviewed Papers


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C4555 Methyl-β-cyclodextrin, powder, BioReagent, suitable for cell culture
92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C
C3045 Cholesterol, powder, BioReagent, suitable for cell culture, ≥99%

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