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  • C5176 - Monoclonal Anti-Cytokeratin Peptide 4 antibody produced in mouse

C5176 Sigma-Aldrich

Monoclonal Anti-Cytokeratin Peptide 4 antibody produced in mouse

clone 6B10, ascites fluid



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytokeratins,
conjugate   unconjugated
clone   6B10, monoclonal
biological source   mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  immunohistochemistry (frozen sections): suitable
  indirect immunofluorescence: 1:300 using formalin-fixed, paraffin-embedded sections of human or animal tissue
  microarray: suitable
  western blot: suitable
species reactivity   feline, sheep, rabbit, hamster, canine, human, guinea pig, goat
mol wt   antigen 59 kDa
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgG1
antibody product type   primary antibodies
contains   15 mM sodium azide
UniProt accession no.   P19013
Gene Information   human ... KRT4(3851)


General description

Cytokeratins are intermediary filaments that are expressed during epithelial development. Cytokeratin-4 is largely found in non-epidermal, stratified epithelial tissues such as larynx, pharynx, vagina, exocervix and epiglottis among several others . Cytokeratin-4 has been implicated in Streptococcus agalactiae infection and genetic mutations in Cytokeratin-4 have been linked to white sponge naevus . Monoclonal Anti-Cytokeratin Peptide 4 antibody is specific for cytokeratin 4 in goats, hamsters, guinea pigs, cats, sheep, humans, rabbits, and dogs. The antibody weakly stains ciliated pseudostratified epithelium of bronchi. Ducts of skin sweat glands and prostate are focally stained by the antibody. Some endocervical columnar cells may also react with the antibody. This antibody is reactive with methanol or acetone fixed frozen sections, and with protease-digested formalin fixed paraffin embedded human tissues. Embedded methacarn fixed material can also be used for cytokeratin 4 demonstration.


The antibody reacts with non-cornifying squamous epithelium (e.g., exocervix, esophagus and cornea). Ciliated pseudostratified epithelium of bronchi is weakly stained. Ducts of sweat glands and prostate are focally stained. The antibody may react with some endocervical columnar cells.


cytokeratin from human esophagus.


Monoclonal Anti-Cytokeratin Peptide 4 antibody is suitable for use in a broad range of immunoassays such as immunohistochemistry (frozen or formalin-fixed, paraffin-embedded sections), immunoblot, dot blot, and indirect immunofluorescence (1:300 using formalin-fixed, paraffin-embedded sections of human or animal tissue). The antibody may also be used for western blot and microarray.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


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