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C5223 Sigma

Anti-Cdk2 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous glycerol solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies for Kinase/Phosphatase Biology,
species reactivity   mouse (predicted), human, rat (predicted)
application(s)   immunoprecipitation (IP): 2-4 μg using 500 μg of HeLa cell nuclear extract
  western blot: 0.5-2 μg/mL using human HeLa cell nuclear extract
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... CDK2(1017)
mouse ... Cdk2(12566)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to amino acids 287-298 (CQDVTKPVPHLRL) of human cdk2.

Physical form

Solution in 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, and 0.05% sodium azide containing 30% glycerol.


The antibody reacts specifically with human cyclin-dependent kinase 2 (Cdk2) (p33cdk2).


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunoprecipitation (1 paper)

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Certificate of Analysis

Certificate of Origin

Safety Information

Personal Protective Equipment  Eyeshields, Faceshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

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