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  • C5301 - Monoclonal Anti-Cytokeratin Peptide 8 antibody produced in mouse

C5301 Sigma

Monoclonal Anti-Cytokeratin Peptide 8 antibody produced in mouse

clone M20, ascites fluid



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytokeratins,
species reactivity   feline, rabbit, bovine, canine, human
application(s)   immunohistochemistry (frozen sections): suitable
  indirect immunofluorescence: 1:200 using frozen sections of human or animal tissue
  western blot: suitable
clone   M20, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 52 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... KRT8(3856)
biological source   mouse
conjugate   unconjugated



cytokeratin from human breast carcinoma cell line MCF7.

General description

Cytokeratin is a 52.5kD cytoplasmic structural protein that belongs to type II, neutral-to-basic subfamily. It consists of 29 different proteins which is the characteristic of epithelial and trichocytic cells. Monoclonal anti-cytokeratin peptide 8 antibody can be used as a marker for epithelial cell differentiation as well as a tool for tumor identification and classification. It can also be used in western blotting. Mouse anti-cytokeratin peptide 8 antibody reacts specifically with various human simple and complex epithelia like- liver, intestine, pancreas, urinary bladder, salivary gland, thyroid, prostate, mesothel, and placenta but not with stratified squamous epithelia. The product also does not react with non-epithelial tissues (exceptions-certain smooth muscle cells). This product has also shown cross reactivity for cytokeratin of rabbit, cow, dog, and cat.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Western Blotting (1 paper)

Monoclonal anti-cytokeratin peptide 8 antibody (diluted 1:100 in PBS) can be used in immunofluorescence microscopy. It can also be used to localize cytokeratin 8 by using many immunochemical assays like immunohistochemistry, immunoblotting and dot blotting.

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Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers


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