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C5338 Sigma

Cyanogen bromide-activated-Sepharose® 4 Fast Flow

lyophilized powder

Synonym: Cyanogen bromide-activated Agarose



Related Categories Activated/Functionalized Matrices, Affinity Chromatography, Molecular Biology, Protein Chromatography, Proteomics More...
form   lyophilized powder
matrix   Sepharose 4 Fast Flow
matrix active group   cyanate or related structures
matrix spacer   1 atom (when ligands are coupled via isourea derivative or related linkage)
swelling   1 g swells to 4-5 mL gel
capacity   6-11 μmol/mL coupling capacity (6-AKS)
storage temp.   2-8°C



1, 5, 10 g in poly bottle

Physical form

Lyophilized powder with sugar stabilizers


Cyanogen bromide-activated-Sepharose® is used in affinity chromatography and protein chromatography. Cyanogen bromide-activated-Sepharose® has been used to discover insecticidal agents, to raise polyclonal antibodies against the yeast invertase glycosyls, and to study immunological processes of xenograft rejection.

Legal Information

Sepharose is a registered trademark of GE Healthcare

Price and Availability

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Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
Do you have a protocol for using Product C5338, Cyanogen bromide?
CNBr-activated Sepharose 4 Fast Flow is a registered trademark of GE Healthcare. A protocol for using this resin can be found on the manufacturer's website: http://www1.gelifesciences.com/aptrix/upp00919.nsf/content/3DC5E0509E2624FEC1256EB400418007?OpenDocument&Path=Catalog&Hometitle=Catalog&entry=4&newrel&LinkParent=C1256FC4003AED40-1FEC142E09A2EE76C12570D9004217B1_RelatedLinksNew-C821BEC677D8448BC1256EAE002E3030&newrel&hidesearchbox=yes&moduleid=166221&zone=
How do I store Product C5338, Cyanogen bromide?
This product as it is supplied, is stored at room temperature.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What does Sepharose 4 Fast Flow mean in the description of Product C5338?
Sepharose 4 Fast Flow is a highly crosslinked 4% agarose matrix which gives excellent physical stability and chromatographic qualities.  It was developed to meet high throughput demands of industrial process separations.  The rigidity of the resin permits high flow rates, which in turn gives good resolution in a minimum amount of time
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Protocols & Articles
Peer-Reviewed Papers


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