• USA Home
  • C5533 - Collagen from human placenta

EMAIL THIS PAGE TO A FRIEND
C5533 Sigma-Aldrich

Collagen from human placenta

Bornstein and Traub Type IV, powder, BioReagent, suitable for cell culture

Synonym: Collagen Type 4

Purchase

Properties

Related Categories Adhesion, Attachment, and Matrix Factors for Stem Cell Expansion, Application Index, Attachment Factors, Attachments Factors, Biochemicals and Reagents,
Quality Level   200
biological source   human placenta
sterility   non-sterile
product line   BioReagent
form   powder
packaging   pkg of 5 mg
application(s)   cell culture | mammalian: suitable
impurities   HIV, hepatitis B and hepatitis C, none detected
solubility   acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... COL4A1(1282), COL4A2(1284), COL4A3(1285), COL4A4(1286), COL4A5(1287), COL4A6(1288)

Description

General description

Type IV collagens are localized to the basement membrane and they are network-forming proteins. It is encoded by six different genes: collagen type IV α1 (COL4A1), COL4A2, COL4A3, COL4A4, COL4A5 and COL4A6. Both COL4A1 and COL4A2 genes are mapped to human chromosome 13q34. The proteins encoded by the two genes share 45% homology. They contain: a non-collagenous (NC1) domain, triple-helical collagenous domain and Gly-Xaa-Yaa repeats in common. Both COL4A3 and COL4A4 genes are located on human chromosome 2q36.3. In tissues, type IV collagens are present as procollagens.

Application

Collagen from human placenta has been used:
• To solubilize extracellular matrix for surface functionalization.
• To induce endothelial cell and rat pluripotent cell differentiation.
• As an adherent (11) in cell migration/invasion assay.

Biochem/physiol Actions

During development, collagen IV is ubiquitously distributed in BMs. During the maturation process, this network gets partially replaced in a remarkably tissue specific manner, defining BM structure and function. Collagen IV has been shown to bind to platelets, hepatocytes, keratinocytes, endothelial, mesangial, pancreatic cells and some tumor cells.


Tissue injury in the autoimmune disease Goodpasture syndrome is due to pathogenic autoantibodies targeting the Collagen IV α3 chain . Mutations in COL4A5 are associated with Alport syndrome.

Type IV collagen maintains cell division and growth. Abnormal collagen secretion affects basement membrane formation. The collagen type IV, α1 (COL4A1) gene is overexpressed in gastric cancer. Mutation in COL4A1 contributes to intracerebral hemorrhage in adults and children. Cerebrovascular disease is resulted due to COL4A1 and COL4A2 mutations.

Components

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type IV collagen occurs only in the basement membranes and contains up to six genetically distinct a-chains.

Caution

This product should be stored desiccated at -20°C, and will retain activity in these conditions for 3 years.

Preparation Note

This is a lyophilized powder that can be reconstituted in sterile .5 M acetic acid, PBS or water at 1 mg/mL. A PBS solution will be stable for at least one year at -20°C. It was prepared by a modification of the pepsin extraction method of Niyibizi, C. et. al

Other Notes

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Single- & multi-channel pipettes from BRAND

Pipette Controller from Brand
Protocols & Articles

Articles

Attachment Factors for 3-Dimensional Cell Culture

Three-dimensional (3D) extracellular matrix-based cell culture systems are being used as research tools for understanding normal and disease systems, and for drug screening in vitro. The extracellula...
Efrat Manaster
BioFiles 2008, 3.8, 16.
Keywords: Adhesion, Angiogenesis, Biomaterials, Cancer, Cell biology, Cell culture, Clinical, Gene expression, Genetic, Genetics, Genomics, Growth factors, Hormones, Molecular biology, Morphogenesis

Cancer Stem Cells: New Targets for Cancer Therapy

Interested in receiving more content like this? Join over 8,000 of your peers who have already signed up for the Cell Informer newsletter.
Keywords: Cancer, Cell attachment, Cell culture, Cell proliferation, Gastrointestinal, Gene expression, Transcription, transformation

Collagen Usage Recommendations

Collagen is one of the most abundant proteins in the connective tissues and internal organs of mammals. It provides the tensile strength of the extracellular matrix (ECM). It is classified into a num...
George Sitterley
BioFiles 2008, 3.8, 4.
Keywords: Cell culture, Morphogenesis

Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
Keywords: Adhesion, Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Cell attachment, Cell culture, Cell proliferation, Coagulation, Endocrinology, Growth factors, Hormones

Overview of 3D Cell Culture: Tools and Techniques

Cell culture techniques are ubiquitous in areas of developmental biology, drug discovery, regenerative medicine and protein production. Since the introduction of cell culture techniques, cells have b...
Keywords: Alkylations, Apoptosis, Biomaterials, Cancer, Cell culture, Cell proliferation, Clinical, Cytotoxins, Drug discovery, Gene expression, Metabolism, Pharmaceutical

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. It has long been understood to be the structural support for cells since its characteristics set the characteristics...
Keywords: Adhesion, Cell culture, Cellular processes, Epimerizations, Growth factors, Immobilization, Sulfations

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?