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C5753 Sigma-Aldrich

Chlorophyll a

from spinach

  • CAS Number 479-61-8

  • Empirical Formula (Hill Notation) C55H72MgN4O5

  • Molecular Weight 893.49

  •  Beilstein/REAXYS Number 3586237

  •  EC Number 207-536-6

  •  MDL number MFCD00079050

  •  PubChem Substance ID 24892803

  •  NACRES NA.47

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Properties

Related Categories Allium sativum (Garlic), Cell Biology, Core Bioreagents, Fucus vesiculosus, Hypericum perforatum (St John′s wort),
Quality Level   200
biological source   spinach
assay   ≥85% (HPLC)
form   powder
impurities   chlorophyll b, essentially free
ε (extinction coefficient)   10.6-11.8 × 104 at 428 nm in diethyl ether (vs. 11.17 × 104, lit.)
  8.2-9.1 × 104 at 662 nm in diethyl ether (vs. 8.63 × 104, lit.)
shipped in   dry ice
storage temp.   −20°C
SMILES string   CCc1c(C)c2cc3c(C=C)c(C)c4cc5nc([C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@@H]5C)c6[C@@H](C(=O)OC)C(=O)c7c(C)c(cc1n2)n([Mg]n34)c67
InChI   1S/C55H73N4O5.Mg/c1-13-39-35(8)42-28-44-37(10)41(24-25-48(60)64-27-26-34(7)23-17-22-33(6)21-16-20-32(5)19-15-18-31(3)4)52(58-44)50-51(55(62)63-12)54(61)49-38(11)45(59-53(49)50)30-47-40(14-2)36(9)43(57-47)29-46(39)56-42;/h13,26,28-33,37,41,51H,1,14-25,27H2,2-12H3,(H-,56,57,58,59,61);/q-1;+2/p-1/b34-26+;/t32-,33-,37+,41+,51-;/m1./s1
InChI key   ATNHDLDRLWWWCB-AENOIHSZSA-M

Description

General description

Chlorophyll a is an important green or blue color pigment in most organisms, which is implicated in oxygenic photosynthesis. It functions as an antenna pigment in photosystems I and II.

Application

A procedure has been described for harvesting native light-harvesting complex IIb (LHCIIb).

Chlorophyll a has been used:
• in the identification of pheophytin a from environmentally friendly cultivated green peppers
• for the high performance liquid chromatography (HPLC) analysis of carotenoid
• to determine specific absorption coefficients of chlorophylls a and b in dimethyl sulfoxide (DMSO) and acetone
• to sense different nitric oxide (NO) species

Packaging

1, 5, 10 mg in ampule

Sealed ampule.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
FW6420000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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C5878 Chlorophyll b from spinach, ≥90% (HPLC), ≤0.5% Chlorophyll a
06693 Timestrip Plus -20 °C

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