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C5788 Sigma-Aldrich

Complement C5a human

recombinant, expressed in E. coli, ~95% (SDS-PAGE), lyophilized powder

Synonym: C5a anaphylatoxin, rC5a

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Properties

Related Categories Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes, Cell Signaling and Neuroscience,
Quality Level   200
biological source   human
recombinant   expressed in E. coli
assay   ~95% (SDS-PAGE)
form   lyophilized powder
application(s)   activity assay: suitable
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... C5(727)

Description

Application

Complement C5a human is an important terminal component of the complement cascade. It is a direct mediator of inflammation, and has been identified as a novel biomarker for pain and inflammation following surgery

Biochem/physiol Actions

C5a, in addition to being a direct mediator of inflammation, can induce both IL-8 (interleukin-8) synthesis and high levels of IL-8 release from monocytes. This secondary effect serves as an amplification mechanism for inflammation at sites of infection or trauma. C5a exerts its effect through a G-protein coupled receptor, CD88.

A mixture of C5a (~35%) and C5a having an added methionyl residue at the amino terminus (~65%); exhibits biological activities similar to serum-derived C5a. C5a is a (11.2kDa) proteolytic fragment of the C5 α-chain through the action of C5 convertases in the classical and alternative complement pathway (C4b2a4b, C3bBb3b). C5a is an anaphylatoxin. It acts as an inflammatory chemoattractant. C5a stimulation of human neutrophils leads to STAT3 phosphorylation on Ser727. It mediates IL-8 release from bronchial epithelial cells. C5a anaphylatoxin activity on hepatocytes results indirectly from interaction with nonparenchymal cell via prostanoid secretion.

Quality

Mol. Wt.: ~8.6 kDa (non-glycosylated, with glutathione attached to cysteine 27).

Other Notes

View more information on the complement pathway at www.sigma-aldrich.com/enzymeexplorer

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Plasma Derived Proteins and Enzymes

Despite their complexity, blood and plasma are abundant biological resources for the discovery of drug targets and biomarkers for human disease. It is estimated that plasma may contain as many as 40,...
BioFiles 2006, 1.5, 2.
Keywords: Applications, Gas chromatography, Type

Peer-Reviewed Papers
15

References

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