• USA Home
  • C5994 - Anti-CAMKK1 (31-45) antibody produced in rabbit

EMAIL THIS PAGE TO A FRIEND
C5994 Sigma

Anti-CAMKK1 (31-45) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-Calcium/calmodulin-dependent protein kinase 1 α isoform b (Homo sapiens), Anti-CAMKKA

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, CAM-CB,
species reactivity   human
application(s)   western blot: 1:500-1:2,000
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt ~57 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CAMKK1(84254)
biological source   rabbit
antibody product type   primary antibodies
conjugate   unconjugated
UniProt accession no.   Q8N5S9

Description

Immunogen

synthetic peptide corresponding to amino acids 31-45 of human CAMKK1.

General description

The gene CAMKK1 (Calcium/calmodulin-dependent protein kinase kinase 1) is mapped to human chromosome 17p13.2. The protein localizes in the cytoplasm and the nucleus.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

CAMKK1 (Calcium/calmodulin-dependent protein kinase kinase 1) is responsible for phosphorylation of Ca2+/calmodulin-dependent protein kinases (CAMKs), CAMK1 and CAMK4. In muscle cells, CAMKK1 is involved in the activation of AMP-activated protein kinase (AMPK) via adiponectin by stimulating Ca2+ release from intracellular stores. Absence of CAMKK1 attenuates phorbol-12-myristate-13-acetate (PMA)-dependent IL (Interleukin)-10 production and enhnaces tumor necrosis factor α (TNFα). These changes cause shift from type-II to classical monocyte activation. CAMKK1 results in AKT (RAC-γ serine/threonine-protein kinase) activation, which inhibits IL-1β-induced nuclear factor-κB (NFκB) activation.

Safety & Documentation

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary and Secondary Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay needs. Use the Antibody...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?