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C6137 Sigma-Aldrich

Chitinase from Streptomyces griseus

lyophilized powder (essentially salt free), ≥200 units/g solid

Synonym: Chitodextrinase, Poly(1,4-β-[2-acetamido-2-deoxy-D-glucoside]) glycanohydrolase



Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis,
Quality Level   PREMIUM
form   lyophilized powder (essentially salt free)
solubility   H2O: soluble 0.90-1.10 mg/mL
storage temp.   −20°C



Chitinase from Streptomyces griseus has been used to study the effect of the allosamidin on the regulatory system for chitinase production. It has also been used to study the enrichment of chitinolytic microorganisms. This enrichment was achieved by the isolation and characterization of a chitinase with antifungal activity against phytopathogenic fungi. The enzyme from Sigma has been used as a standard for chitinase assays using the enzyme derived from Absidia glauca and Petunia hybrida extracts.


5, 25, 50 units in glass bottle

Biochem/physiol Actions

Chitinase is an extracellular enzyme complex that degrades chitin and has a molecular mass of approximately 30 kDa. Chitin is degraded to N-acetyl-D-glucosamine in 2 enzymatic reactions. Firstly, chitobiose units are removed from chitin by chitodextrinase-chitinase. The second reaction involves N-acetyl-glucosaminidase-chitobiase, which cleaves the disaccharide to its monomer subunits (that comprise of N-acetyl-D-glucosamine). The optimum reaction temperature is 37 °C.

Unit Definition

One unit will liberate 1.0 mg of N-acetyl-D-glucosamine from chitin per hour at pH 6.0 at 25 °C in a 2 hour assay.
One new 1 hour unit = approx. 50 old 48 hour units.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis


Certificate of Origin

Protocols & Articles

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