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C6158 Sigma-Aldrich

Crystal Violet

ACS reagent, ≥90.0% anhydrous basis

Synonym: Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B

  • CAS Number 548-62-9

  • Linear Formula C25H30N3Cl

  • Molecular Weight 407.98

  •  Colour Index Number 42555

  •  Beilstein/REAXYS Number 3580948

  •  EC Number 208-953-6

  •  MDL number MFCD00011750

  •  PubChem Substance ID 57654029

  •  NACRES NA.47

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Properties

Related Categories C, Cell Biology, Hematology and Histology, Stains & Dyes, A to Z, Stains and Dyes More...
Quality Level   200
grade   ACS reagent
assay   ≥90.0% anhydrous basis
form   powder
application(s)   hematology: suitable
  histology: suitable
loss   ≤7.5% loss on drying
mp   205 °C (dec.) (lit.)
solubility   water: soluble 50 g/L at 27 °C
density   1.19 g/cm3 at 20 °C
absorption   passes test
suitability   passes test for sensitivity as indicator
Featured Industry   Diagnostic Assay Manufacturing
Mode of action   cell membrane | interferes
  enzyme | inhibits
antibiotic activity spectrum   fungi
storage temp.   room temp
SMILES string   [Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C
InChI   1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1
InChI key   ZXJXZNDDNMQXFV-UHFFFAOYSA-M

Description

Application

Crystal violet has been used to determine cell viability. It has been used in the staining of cells for the quantification of cellular migration.

Packaging

50, 100 g in glass bottle

Biochem/physiol Actions

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal violet helps in colorimetric measurement of cell viability.

Safety & Documentation

Safety Information

Signal word 
Danger
Hazard statements 
RIDADR 
UN 3077 9 / PGIII
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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